Obiettivo
It is common knowledge that an ethernet connection is safer than a wireless connection, and cellular organelles share this approach. To communicate directly among each other, organelles are thought to use Membrane Contact Sites (MCS), the optical fibers at the intracellular level. Organelles are in a prime position to sense and communicate stress signals due to their tight integration into the cells metabolic networks. Inter-organelle communication is thus crucial to pass on the message to coordinate cellular stress responses and maintain homeostasis. I hypothesise that MCS allow fast and efficient communication of stress signals between intracellular organelles (e.g. mitochondria and ER) to coordinate the cellular stress responses. However, the organelle tethering proteins, the stimuli driving MCS formation and the signalling molecules mediated through these MCS remain elusive in plants.
In INTERCOM, I will characterise ER-mitochondria communication in response to stress in Arabidopsis thaliana. For that, I will leverage the host labs previously established ER-mitochondria communication model system and establish a proteomic screening setup to identify novel proteins involved in ER-mitochondria MCS in plants. In addition, I will develop a high-throughput platform to study inter-organelle interactions in vivo and to identify stimuli driving MCS dynamics. Finally, I will employ genetically encoded biosensors to pinpoint ROS and calcium inter-organelle signalling events. To reach my goal, I will combine my expertise in intracellular signalling and bioimaging with cutting-edge interactomics, live-cell imaging and high-content technologies available at the host institute. This innovative and interdisciplinary approach will allow me to shed light on plant inter-organelle communication, a field still in its infancy in plant biology with the potential to pave the path for the biotechnological engineering of plants more resilient to harmful environmental conditions.
Campo scientifico (EuroSciVoc)
CORDIS classifica i progetti con EuroSciVoc, una tassonomia multilingue dei campi scientifici, attraverso un processo semi-automatico basato su tecniche NLP.
CORDIS classifica i progetti con EuroSciVoc, una tassonomia multilingue dei campi scientifici, attraverso un processo semi-automatico basato su tecniche NLP.
- ingegneria e tecnologiaingegneria elettrica, ingegneria elettronica, ingegneria informaticaingegneria elettronicasensoribiosensori
- ingegneria e tecnologiaingegneria dei materialifibre
- scienze naturaliscienze chimichechimica inorganicametalli alcalino terrosi
- scienze naturaliscienze biologichebiochimicabiomolecoleproteine
- scienze mediche e della salutemedicina di basefisiologiaomeostasi
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Parole chiave
Programma(i)
- HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA) Main Programme
Meccanismo di finanziamento
HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European FellowshipsCoordinatore
9052 ZWIJNAARDE - GENT
Belgio