Objective
Human microbiome science is advancing, showing promise of having important impacts on human health with new insights into disease etiology and general human biology. Accordingly, DNA sequencing platforms have been developed rapidly, expanding the scope in microbiome research. Among others, optical mapping technique, capable of imaging single DNA molecules provides access to genetic information on single molecules up to ~1 Mbp in length. It uses fluorescence imaging of linearly stretched DNA molecules labelled at specific sites to probe information patterns along the molecules. The precision of the method is further improved by resolving single molecules below the diffraction limit through super-resolution imaging. However, requirement of large photon budget slows down the speed of imaging, as long acquisitions are required to build up sufficient photons for precise localization. This affects the throughput of DNA optical mapping which involves imaging large areas, thereby limiting its capabilities in providing real-time information of the microbiome on daily basis. Enhancing the emission rates from single molecules can overcome this limitation to permit faster image acquisition. Here we propose to use plasmon-enhanced fluorescence by employing substrate made out of wet-chemically synthesized gold nanotriangles for optical mapping of human gut microbiome. Plasmonic nanostructures can confine incident electromagnetic field into small area near their surface leading to strong light absorption and higher emission rates from fluorophores in their vicinity. The use of wet-chemical plasmonic substrate exhibiting strong plasmon resonance can significantly increase the number of photons released from the labelled DNA, allowing faster image acquisition. With the improved speed, the time required for optical mapping analysis can be significantly shortened, enabling longitudinal metagenomic analysis with practical implications for quicker diagnosis and personalized health care.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences physical sciences condensed matter physics quasiparticles
- natural sciences biological sciences genetics DNA
- natural sciences physical sciences optics microscopy super resolution microscopy
- natural sciences biological sciences microbiology
- natural sciences physical sciences theoretical physics particle physics photons
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA)
MAIN PROGRAMME
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) HORIZON-MSCA-2022-PF-01
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
3000 LEUVEN
Belgium
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.