Project description
DNA beads for the future of flexible data storage
Data storage technologies are rapidly advancing, yet current digital solutions face limitations in stability and capacity. DNA holds potential as a high-density storage medium, but its practical use is hindered by challenges in stable data encoding and retrieval. Existing methods either offer static storage or lack effective in vivo manipulation. Addressing these issues, the EIC-funded DURA-store project has designed an approach using a new ‘regeneratable’ solid-state storage system consisting of beads. This system encodes data in single-stranded DNA, enabling selective addition, access, and deletion through enzymatic reactions. Unique Data ID sequences facilitate precise data operations, while RNA molecules and bacteriophages enhance data access and management in bacterial models. The integration of damage-suppressing proteins from extremophiles further ensures long-term stability, advancing the feasibility of DNA-based data storage.
Objective
In order for DNA to become the information storage medium that serves as an alternative to existing digital storage solution technologies information must be stored stably in it with the means to repeatedly access and manipulate parts of the stored data. Only few in vitro approaches are capable to address some of these requirements, while in vivo approaches still produce largely static data storage libraries, which limits the real-life applicability of these technologies. In this proposal we present a new, regeneratable solid state storage system consisting of beads, where information, encoded in single stranded DNA strands, can be added, selectively accessed and removed using enzymes and nucleic acid strands as inputs for the different data operations in isothermal reactions with no loss of material. To achieve this a strand architecture is proposed, where unique Data ID sequences are used on the targeted data strands for performing the intended data operations via enzymatic reactions: addition, deletion of data and transcription-based data access. Furthermore, a variant of this system is proposed that uses RNA molecules for the selective access of the data strands, which we aim to implement in a bacterial data storage system as well where RNA encoding bacteriophages will be used as a non-invasive way to introduce inputs for the data operations, such as random data access and removal. Finally, we present the use of damage suppressor proteins from extremotolerant organisms complementing the in vitro and in vivo system to provide the long-term stability of DNA observed in resilient biological systems.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques.
- natural sciencesbiological sciencesbiochemistrybiomoleculesnucleic acids
- natural sciencescomputer and information sciencesdatabases
- natural sciencesbiological sciencesgeneticsDNA
- natural sciencesbiological sciencesgeneticsRNA
- natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes
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Programme(s)
- HORIZON.3.1 - The European Innovation Council (EIC) Main Programme
Funding Scheme
HORIZON-EIC - HORIZON EIC GrantsCoordinator
17177 Stockholm
Sweden