Project description
Paving the way for precision gene therapy
A multitude of genetic disorders have long challenged the medical community, yet direct intervention at the genetic level through gene therapy remains limited. The stumbling blocks are complex delivery routes and the instability of therapeutic nucleic acids. Funded by the European Research Council, the POLYPATH project will address these gaps, aiming to unlock the full potential of gene therapy. Polyplexes, tiny complexes of nucleic acids and synthetic polymers, offer a promising solution. Despite years of research, their potential remains untapped due to ill-defined formation routes and structural variability. POLYPATH’s results will result in the creation of a library of well-defined structures, with mechanisms studied using cutting-edge technology. The overall goal is to bring functional polyplexes to the forefront of genetic medicine.
Objective
A vast number of pathologies have a genetic origin. Yet, using gene therapy to directly intervene at the genetic root remains a rarity within today’s pharmaceutical arsenal. The main hurdles in promoting gene therapy from the lab to the clinic are the complex delivery pathways and biological instability of the therapeutic nucleic acids. Polyplexes, nanoscale coacervates of nucleic acids and (synthetic) polymers, hold the prospect of being highly tunable, scalable and robust transport vehicles and as such a key enabler for gene therapy.
Despite years of active research, however, polyplexes have yet to fulfil their claimed potential. Our understanding of polyplex formation and the followed assembly pathways are presently insufficient for the rational design of efficient and selective gene delivery vehicles. Prototypical polyplex formation routes are ill-defined, yielding a broad spectrum of unequilibrated structures. The effect of this structural polydispersity on gene delivery and transfection efficiency is unknown, critically hampering the potency of polyplexes.
The aim of POLYPATH is to develop polyplex fabrication routes via controllable and predictable assembly pathways. These routes rely on the in situ growth of the encapsulating polymers in the presence of the nucleic acids and yield temporal, on-demand control of the attractive interactions that drive polyplex formation. With this synthetic control, we will create a systematic, predictable library of structurally well-defined polyplexes. The assembly processes will be elucidated with state-of-the-art time-resolved X-ray scattering and spectrally-resolved NMR relaxometry and diffusometry. To bridge the knowledge gap between polyplex structure and function, we will use fluorescence correlation spectroscopy to directly measure polyplex stability and fate in cellular environments. Ultimately, POLYPATH will provide mechanistic insights that can finally bring functional polyplexes towards the clinic.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences chemical sciences polymer sciences
- natural sciences physical sciences optics spectroscopy
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Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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HORIZON.1.1 - European Research Council (ERC)
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
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Call for proposal
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(opens in new window) ERC-2023-STG
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3584 CS Utrecht
Netherlands
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