SHIELD4GRAPE’s objectives will be achieved by applying approaches specifically tailored to the different resilient genotypes.
Exploitation of grapevine biodiversity and identification of new traits for resilience. The most interesting resilient accessions were characterized morphologically and genetically to identify key tolerance-associated traits. The interaction between pathogens and these resilient accessions were analysed by integrating different Omics.
Breeding activities. The first strategy encompassed the implementation of traditional breeding activities on local varieties. The second one relied on the New Genomics Techniques (NGTs), which can represent the future of genetic improvement in plants with the production of cisgenic and edited grapevines. The third breeding approach addressed in S4G was the induction of mutations in embryogenic tissues and regeneration of plants (somaclones) with improved tolerance to environmental stresses.
Field trials and IPM. S4G associated selected resilient genotypes with application of specific IPM strategies. Various IPM strategies were applied to different genotypes in different experimental/demonstrative vineyards. The effect of diverse IPM protocols was tested using multi- and transdisciplinary approaches. The preservation of beneficial endophytes and pollinators will be considered, and fungicide residues will be evaluated.
Socio-economic tools. The impact of field-tested innovative approaches was evaluated by using a holistic multi-criteria methodology that integrated environmental, economic and social tools/indexes. Semi-structured interviews and surveys by questionnaire were developed to collect primary and representative data about current management practises, drivers and barriers in the adoption of new/rediscovered grapevine genotypes
Knowledge networking. The S4G consortium invested efforts to dissemination and demonstration activities, targeting researchers, students, farmers, producers, agricultural advisors, consumers, and policy makers. These actions spread knowledge among the different actors by sharing the science outcomes and best practises across the diverse agro-ecosystems.