Periodic Reporting for period 1 - SHIELD4GRAPE (BREEDING AND INTEGRATED PEST MANAGEMENT STRATEGIES TO REDUCE RELIANCE ON CHEMICAL PESTICIDES IN GRAPEVINE)
Okres sprawozdawczy: 2024-02-01 do 2025-07-31
The overall objective of SHIELD4GRAPE (S4G) is to implement sustainable agroecological approaches contributing to the improvement of resilience of the grapevine agri-food systems against pest diseases in a context of climatic changes. S4G will i) exploit grapevine biodiversity and identify new resilience traits; ii) implement breeding activities, including new breeding techniques and mutagenesis; iii) introduce safer and more sustainable strategies against pathogens. Demonstration fields will be set up in all the most important EU regions interested in viticulture to improve the efficacy of applied integrated pest management protocols in combination with the new resilient genotypes. S4G aims to support farmers and researchers, to provide advisory services and actors in value chains by establishing an interrelated community that will facilitate exchange of knowledge and of the best practices at different levels and socio-economic resilience. S4G will contribute to safeguard the territory, human health, farmers, the population living in grape-growing areas and consumers.
Exploitation of grapevine biodiversity and identification of new traits for resilience. The most interesting resilient accessions were characterized morphologically and genetically to identify key tolerance-associated traits. The interaction between pathogens and these resilient accessions were analysed by integrating different Omics.
Breeding activities. The first strategy encompassed the implementation of traditional breeding activities on local varieties. The second one relied on the New Genomics Techniques (NGTs), which can represent the future of genetic improvement in plants with the production of cisgenic and edited grapevines. The third breeding approach addressed in S4G was the induction of mutations in embryogenic tissues and regeneration of plants (somaclones) with improved tolerance to environmental stresses.
Field trials and IPM. S4G associated selected resilient genotypes with application of specific IPM strategies. Various IPM strategies were applied to different genotypes in different experimental/demonstrative vineyards. The effect of diverse IPM protocols was tested using multi- and transdisciplinary approaches. The preservation of beneficial endophytes and pollinators will be considered, and fungicide residues will be evaluated.
Socio-economic tools. The impact of field-tested innovative approaches was evaluated by using a holistic multi-criteria methodology that integrated environmental, economic and social tools/indexes. Semi-structured interviews and surveys by questionnaire were developed to collect primary and representative data about current management practises, drivers and barriers in the adoption of new/rediscovered grapevine genotypes
Knowledge networking. The S4G consortium invested efforts to dissemination and demonstration activities, targeting researchers, students, farmers, producers, agricultural advisors, consumers, and policy makers. These actions spread knowledge among the different actors by sharing the science outcomes and best practises across the diverse agro-ecosystems.
SHIELD4GRAPE breaks through the state of the art by investigating novel breeding strategies and IPM to boost the development of new grapevine varieties with improved tolerance to relevant diseases and better adapted to local environmental conditions.
The progress beyond the state-of-the-art:
In S4G, the resilience of minor/local/neglected varieties to biotic factors was evaluated using leading technology, and the most interesting genotypes were characterized.
S4G has been identifying new resilience traits starting from the high genetic variability available in the Consortium and focusing on traits associated to general tolerance to multiple diseases by integrating multi-Omics approaches.
S4G has increased the availability of new grapevine genotypes resilient to pest diseases using different techniques to fully satisfy the needs of all EU territories, rules and socio-cultural contexts.
S4G developed tailored SynComs to prime grapevines against environmental stresses. S4G has been implementing different IPM strategies, specific for the target grapevine genotypes (local varieties, resistant hybrids, somaclones), and has been validating protocols in different EU biogeographical regions.