Description du projet
Diagnostic des polluants biotiques et abiotiques à l’aide de biocapteurs
La détection des polluants abiotiques et biotiques est cruciale pour les applications environnementales. Le projet BIOSENSEI, financé par l’UE, vise à développer une plateforme de biocapteurs qui utilise les réponses cellulaires pour détecter les deux types de polluants. Ces biocapteurs seront encapsulés et immobilisés sur des transducteurs bimodaux, ce qui permettra une détection extrêmement sensible et fiable des polluants ciblés. En outre, le projet créera des frontaux analogiques à très faible consommation et des nœuds finaux IdO autonomes pour l’acquisition de données, ce qui facilitera l’intégration dans les réseaux LoRa existants et fournira des flux de données en temps réel. Un tableau de bord en ligne permettra aux utilisateurs finaux de visualiser les données. Mené dans le cadre d’une conception sûre et durable, le projet contribuera de manière significative à la vision «zéro pollution» de l’UE.
Objectif
BIOSENSEI develops a real-time, multiplexed, end-to-end, tailored and reliable biosensor platform, using cellular responses, for detection of abiotic pollutants - Nutrients, Estrogenic endocrine-disrupting chemicals, and PFAS (Perfluoroalkyl and Polyfluoroalkyl Substances); and biotic pollutants - Microcystins. Cellular biosensors from bacterial variants will be genetically engineered using, RNA-RNA interactive and type III CRISPR-Cas-mediated transduction cascades. These biosensors are encapsulated and immobilised at bi-modal transducers (nanoelectrochemical and optical) to provide highly reliable, tuneable and sensitive detection of the target pollutants. Bespoke ultra-low power analog front ends and autonomous IoT end-nodes will enable operation and data acquisition from biosensors and facilitate easy integration in existing LoRa networks enabling real-time data feeds. Neural computing algorithms are embedded on the edge to correct for sensor aging and interferents in the (bio)chemical transduction and improve sensor data accuracy. An online dashboard will be developed to allow end users to visualize data. BIOSENSEI will embed the whole R&D process within a safe-and-sustainable-by-design framework to guarantee environmental safety related to risks of potential release into the open environment. Biosensors will be scalable, adaptable to different applications in water & soil and will be deployed in four different use-cases. The consortium is vertically integrated bringing expertise in cellular biology, surface chemistry, nanoelectronics fabrication, hardware integration, regulatory and industrial sampling and artificial intelligence. BIOSENSEI directly addresses HORIZON-CL6-2023-ZEROPOLLUTION-01-6 Biosensors and user-friendly diagnostic tools for environmental services and will allow cellular biosensors to be deployed outside laboratory settings for the first time the project and has the potential to considerably contribute to fulfil EU vision on zero-pollution.
Champ scientifique
- natural sciencescomputer and information sciencesartificial intelligence
- engineering and technologyelectrical engineering, electronic engineering, information engineeringelectronic engineeringsensorsbiosensors
- natural sciencescomputer and information sciencesinternetinternet of things
- engineering and technologynanotechnologynanoelectronics
Mots‑clés
Programme(s)
Régime de financement
HORIZON-RIA - HORIZON Research and Innovation ActionsCoordinateur
T12 YN60 Cork
Irlande