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Molecular mimicry and immune evasion in malaria parasites

Objective

Parasites have evolved elaborate strategies that maximise their fitness. Central to these strategies is their interaction with the host immune system, for instance with immune receptors which mediate pathogen recognition or immune tolerance. Human malaria parasites encode a family of ~200 rifins, some of which are molecular mimics of human self-identifying proteins and that bind tolerance-inducing receptors. However, very few host targets of rifin proteins are known. Our data suggests that the family at large may form an elaborate repertoire of immunomodulatory effectors. We postulate that rifins are deployed to manipulate host immunity in order to facilitate parasite persistence in its host. We further hypothesise that this immune regulation is underpinned by a complex specificity code and a distinct bet-hedging expression program. In the Trojan project, we will investigate and contextualise immunomodulation by malaria parasites in the arms race between the parasite and its host. Firstly, given the sequence diversity of rifins, we will optimise a method to measure gene expression without the need for a reference genome on a single cell level. We will then deploy this tool to understand how expression of rifins is associated with malaria symptoms and persistence in long-term natural infections. Secondly, we will decipher the range of immune targets of rifins as well as the rifin-specificity code and use lineage tracing to understand how diverse immunomodulatory expression patterns arise. Finally, we will seek to understand how malaria-infected individuals respond to immunomodulation by interfering with rifin-receptor interactions. Altogether we will reveal how pathogen molecular mimicry and manipulation is central to their success. This study will transform our understanding of fundamental aspects of parasite biology as well as immune homeostasis and could provide new tools in the continuing fight against malaria.

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Keywords

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Programme(s)

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Topic(s)

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Funding Scheme

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HORIZON-ERC - HORIZON ERC Grants

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Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

(opens in new window) ERC-2023-ADG

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Host institution

INSTITUT DE RECHERCHE POUR LE DEVELOPPEMENT
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 2 499 923,75
Address
BOULEVARD DE DUNKERQUE 44 CS 90009
13572 Marseille
France

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Region
Provence-Alpes-Côte d’Azur Provence-Alpes-Côte d’Azur Bouches-du-Rhône
Activity type
Research Organisations
Links
Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 2 499 923,75

Beneficiaries (1)

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