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Next-generation fungicides for crop protection based on artificial small RNAs derived from viral vectors

Project description

Combatting crop fungi with new RNA-based solutions

Plants face an escalating threat from fungal pathogens, leading to significant crop losses globally. Despite widespread fungicide use, these losses persist, exacerbated by climate change and the rise of fungicide-resistant strains. Traditional RNA interference tools, while effective, lack specificity, hindering targeted antifungal resistance. Supported by the Marie Skłodowska-Curie Actions (MSCA) programme, the FunSynVIGS project pioneers a GMO-free approach that uses viral vectors to deliver highly specific artificial small RNAs, potentially revolutionising crop protection against pathogens like Botrytis cinerea. Through optimisation in Nicotiana benthamiana and subsequent application in tomato plants, the project aims to revolutionise crop protection strategies. Success promises a new era of environmentally friendly, highly efficient fungicides, securing both current and future food sources.

Objective

Plants are threatened by a plethora of pathogens, with fungi accounting for important crop and post-harvest losses worldwide. Despite widespread spraying of fungicides, losses keep rising in the current warming world and the increasing use of chemical (and toxic) fungicides favours the emergence of resistant fungi strains. Hence, there is an urgent need to develop a new generation of highly efficient and environmental-friendly fungicides for securing present and future food availability.
Classic RNA interference (RNAi) tools based on double-stranded RNA have been used for inducing antifungal resistance in plants, despite their lack of high specificity. More modern and highly specific RNAi tools are based on artificial small RNAs (art-sRNAs). Art-sRNA tools have been optimized mainly for high efficacy and transgenic use but are not well adapted for GMO-free application and have not been used yet for inducing antifungal resistance in plants.
FunSynVIGS seeks to develop a novel, GMO-free RNAi technology consisting in systemic, viral vector-based fungicides expressing art-sRNAs for plant immunization against pathogenic fungi. The specific objectives are:
(1) The development and optimization of viral vector-based fungicides expressing art-sRNAs against Botrytis cinerea, the causal agent of the gray mold disease, in the model plant species Nicotiana benthamiana.
(2) The application of viral vector-based fungicides for immunizing tomato plants against B. cinerea.
Successful completion of these objectives will provide a novel class of mobile RNAi-based fungicides for inducing highly efficient antifungal resistance in crops.

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HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships

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Call for proposal

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(opens in new window) HORIZON-MSCA-2023-PF-01

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Coordinator

AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 181 152,96
Address
CALLE SERRANO 117
28006 MADRID
Spain

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Comunidad de Madrid Comunidad de Madrid Madrid
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Research Organisations
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