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Towards greener and more sustainable analytical approaches: Development of an integrative supercritical CO2-based multidimensional system for bioactive compound study in natural and biological samples

Project description

Environmentally friendly sample analysis

Carbon dioxide that is in a state above its critical temperature and pressure is known as supercritical carbon dioxide. In this state, carbon dioxide exhibits unique properties and versatility that make it an important tool in various industrial and scientific applications, particularly where traditional solvents pose safety or environmental concerns. Funded by the Marie Skłodowska-Curie Actions programme, the GREENMULTIDIMCO2 project aims to advance an existing system that combines supercritical fluid extraction and chromatography for the analysis of plant-based compounds. The key objective is to explore the applicability and scope of the system for other bioactive compounds.

Objective

Chemical characterization is essential to the study of bioactive compounds in natural and biological samples but conventional preparative and analytical techniques are usually time, energy and solvent-intensive, posing environmental and health risks. Supercritical carbon dioxide (CO2) present an eco-friendly alternative due to its interesting physio-chemical properties. Prof. Wests research group has been developing approaches involving supercritical CO2 for two decades, and recently developed an integrated system combining supercritical fluid extraction (SFE) and supercritical fluid chromatography (SFC) for the on-line extraction and analysis of polar and non-polar bioactive compounds from plants. Although feasible, such coupling is very challenging, especially when targeting compounds with a wide range of polarities. The next step is thus to further improve the developed system and explore its applicability to other samples with different matrices and classes of compounds. Specifically, the use of unified chromatography (UC), an advanced version of SFC, will be investigated to further expand the efficiency and potential scope of the system. Since the interfacing of the two techniques is a major bottleneck preventing the system from reaching its full potential, finding innovative solutions to facilitate the extract transfer from the first to the second dimension is necessary. An important part of this project will thus be devoted to investigating new interface modalities to address the compatibility problems and instrumental issues pertaining to this hyphenation. To boost chromatographic separation power and facilitate structural elucidation, the potential of incorporating multidimensional (mD) separation steps while coupling the system with mass spectrometry (MS) will also be explored, to develop a novel on-line (mD-SFE)-(mD-SFC/UC)-MS system. Finally, the systems utility will be demonstrated through qualitative and quantitative analyses of real-life samples.

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Programme(s)

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Topic(s)

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Funding Scheme

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HORIZON-TMA-MSCA-PF-GF - HORIZON TMA MSCA Postdoctoral Fellowships - Global Fellowships

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Call for proposal

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(opens in new window) HORIZON-MSCA-2023-PF-01

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Coordinator

UNIVERSITE D'ORLEANS
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 301 660,80
Address
CHATEAU DE LA SOURCE
45067 Orleans Cedex 2
France

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Region
Centre — Val de Loire Centre — Val de Loire Loiret
Activity type
Higher or Secondary Education Establishments
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Total cost

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Partners (3)

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