Descripción del proyecto
Análisis de muestras respetuosos con el medio ambiente
El dióxido de carbono que se encuentra en un estado superior a su temperatura y presión críticas se conoce como «dióxido de carbono supercrítico». En este estado, el dióxido de carbono presenta propiedades únicas y una versatilidad que lo convierten en una herramienta importante en diversas aplicaciones industriales y científicas, sobre todo cuando los disolventes tradicionales plantean problemas de seguridad o medioambientales. Financiado por las acciones Marie Skłodowska-Curie, el equipo del proyecto GREENMULTIDIMCO2 pretende avanzar en un sistema ya existente que combina la extracción con fluidos supercríticos y la cromatografía para el análisis de compuestos de origen vegetal. El objetivo clave es explorar la aplicabilidad y el alcance del sistema para otros compuestos bioactivos.
Objetivo
Chemical characterization is essential to the study of bioactive compounds in natural and biological samples but conventional preparative and analytical techniques are usually time, energy and solvent-intensive, posing environmental and health risks. Supercritical carbon dioxide (CO2) present an eco-friendly alternative due to its interesting physio-chemical properties. Prof. Wests research group has been developing approaches involving supercritical CO2 for two decades, and recently developed an integrated system combining supercritical fluid extraction (SFE) and supercritical fluid chromatography (SFC) for the on-line extraction and analysis of polar and non-polar bioactive compounds from plants. Although feasible, such coupling is very challenging, especially when targeting compounds with a wide range of polarities. The next step is thus to further improve the developed system and explore its applicability to other samples with different matrices and classes of compounds. Specifically, the use of unified chromatography (UC), an advanced version of SFC, will be investigated to further expand the efficiency and potential scope of the system. Since the interfacing of the two techniques is a major bottleneck preventing the system from reaching its full potential, finding innovative solutions to facilitate the extract transfer from the first to the second dimension is necessary. An important part of this project will thus be devoted to investigating new interface modalities to address the compatibility problems and instrumental issues pertaining to this hyphenation. To boost chromatographic separation power and facilitate structural elucidation, the potential of incorporating multidimensional (mD) separation steps while coupling the system with mass spectrometry (MS) will also be explored, to develop a novel on-line (mD-SFE)-(mD-SFC/UC)-MS system. Finally, the systems utility will be demonstrated through qualitative and quantitative analyses of real-life samples.
Palabras clave
Programa(s)
- HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA) Main Programme
Régimen de financiación
HORIZON-TMA-MSCA-PF-GF - HORIZON TMA MSCA Postdoctoral Fellowships - Global FellowshipsCoordinador
45067 Orleans Cedex 2
Francia