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Deciphering subsurface carbon fixation

Project description

How subsurface microbes affect the global carbon cycle

Earth’s subsurface remains largely uncharted territory for biogeochemistry and microbial ecology despite containing the vast majority of carbon. While surface carbon fixation has been extensively studied, subsurface carbon fixation remains poorly understood. Recent studies have begun to shed light on subsurface carbon-fixing organisms but are isolated and do not accurately describe the geoenvironmental contexts that influence these metabolisms. Funded by the Marie Skłodowska-Curie Actions programme, the SUBCARB project aims to enhance understanding of how subsurface conditions influence the global carbon cycle. Researchers will combine metagenomic and metaproteomic with controlled culturing experiments to explore environmental factors and validate metabolic versatility. Integrating these results with stable isotope analysis should help build a model to predict carbon fixation fluxes.

Objective

Despite containing the vast majority of carbon on the planet, the Earth’s subsurface represents a mostly uncharacterized frontier for biogeochemistry and microbial ecology. While carbon fixation at the Earth’s surface has been drawing a lot of attention for decades, subsurface carbon fixation remains poorly understood. Yet, metabolically, it engages a rich diversity of non canonical pathways, contrasting to surface carbon fixation dominated by the canonical Calvin cycle. Also, its contribution to the global carbon cycle, though long overlooked, is now garnering increased attention. In recent years, several studies aiming to characterise the diversity of carbon-fixing organisms within the subsurface have emerged. However, these studies are isolated and do not accurately describe the dependence of these metabolisms on the geoenvironmental context. I propose here to provide a blueprint of subsurface carbon fixation on Earth. In a top-down approach, I will systematically sample various subsurface environments for metagenomic and metaproteomic analysis of carbon fixing metabolisms, in search of the main environmental factors determining the carbon fixation strategy used. In parallel to this, I will undertake a bottom-up experimental approach to explore and confirm this metabolic versatility in response to changing environments, through controlled culturing experiments. Finally, I will integrate the acquired results with stable isotope analysis to build a metabolic model, aiming to comprehensively predict carbon fixation fluxes in function of the geoenvironmental context. The proposed holistic approach will increase our understanding of the role of subsurface microbial metabolisms in global carbon cycling.

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HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships

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Call for proposal

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(opens in new window) HORIZON-MSCA-2023-PF-01

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Coordinator

UNIVERSITA DEGLI STUDI DI NAPOLI FEDERICO II
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 172 750,08
Address
CORSO UMBERTO I, 40
80138 Napoli
Italy

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Region
Sud Campania Napoli
Activity type
Higher or Secondary Education Establishments
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Total cost

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