A synthesis laboratory was established, supported by the recruitment of a student assistant. Key equipment including a vacuum pump with pressure control and a microwave reactor was installed, enabling a fivefold increase in production scale. Reaction conditions and work-up procedures for both monomer and polymer were optimized for reproducibility. Characterization methods were implemented, and a protocol database was created. An optimal matrix was identified using standard protocols, and iterative feedback between synthesis, biology, and product development has already improved polymer performance.
In parallel, significant progress was made in the development of the platform technology consumable. The modular design was refined through prototyping and simulation, optimizing it for injection molding and standardization. A major step was the redesign from additive manufacturing to formats compatible with common lab tools, such as well plates and microscope slides. Injection molding simulations were performed, and initial testing with a benchtop machine was conducted.
Scaffold production was transitioned from additive manufacturing to vacuum casting, improving scalability, cost-efficiency, and reproducibility. This method enables the generation of biologically relevant structures in a production-stable manner. External testing with academic partners is ongoing.
The first prototypes resembling the envisioned final product were produced and characterized for biomedical usability by testing several matrix formulations at varying concentrations. A key outcome was the formation of a confluent endothelial layer, confirmed by immunofluorescence staining and functional validation to assess barrier function and open, perfusable channels. This indicates proper cell-cell contact and cytoskeletal integrity, consistent with a mature endothelial phenotype.
These results are supported by accompanying protocols and manuals. Samples were distributed to initial partners and pilot users for testing and feedback. These developments lay the foundation for subsequent internal product iterations and market preparation.
