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Protecting the thymic epithelial cells and thymopoiesis during hematopoietic stem cell transplantation

Project description

Protecting the thymus during allogeneic haematopoietic stem cell transplantation

Allogeneic haematopoietic stem cell transplantation (alloHSCT) can cause significant damage to the thymus, impairing its ability to regenerate functional T cells. This is often exacerbated by conditioning regimens and graft-versus-host disease, leading to increased susceptibility to infections and malignancies. The ERC-funded ProtecTHY project aims to protect the thymus from alloHSCT-mediated damage by studying its intrinsic protective mechanisms. The research team will employ an innovative whole organ culture system and a unique collection of human thymus samples to identify how alloHSCT disrupts its structure and assess whether existing clinical treatments can restore thymopoiesis. Findings will pave the way towards new strategies for improved alloHSCT outcomes.

Objective

Allogeneic hematopoietic stem cell transplantation (alloHSCT) is a common, often last-resort treatment for haematological diseases, but it is plagued by long-term morbidity and high mortality. Direct alloHSCT-induced damage to the thymus and subsequent loss of thymopoiesis, ie the production of new T cells, is considered one of the main reasons for these complications. Our limited understanding of how the thymus is damaged during alloHSCT restricts the development of thymus-protecting protocols. The goal of ProtecTHY is to unravel the intrinsic protective mechanisms of the human thymus and assess how alloHSCT affects them, with the ultimate objective of devising measures to prevent thymic damage. We take advantage of an innovative whole organ culture system for the human thymus developed in my laboratory and our extensive pre-existing collection of human thymus tissue samples spanning a diverse age range. ProtecTHY specifically aims to 1) discover how the thymus protects itself from external insults over the course of the human lifetime, 2) reveal how alloHSCT disrupts the human thymus microanatomy and how that damage can be prevented with a cellular therapy, and 3) examine how thymic damage affects the circulating true naive T cells and if existing clinical treatments for alloHSCT complications can restore the loss of thymopoiesis. We will apply state-of-the-art spatial and single-cell multiomics to dissect these protective layers of the thymus both ex vivo and after alloreactive challenge in vitro. Moreover, by testing the effect of treatments already in clinical use, ProtecTHY can resolve their specific mechanisms of action and impact on the thymus. Our results are thus directly translatable to the human condition and can direct future treatments to enhance outcomes following alloHSCT. As a translational immunologist and clinical microbiologist with extensive experience in human thymus research, I am well equipped to uncover the mechanisms of thymic protection.

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HORIZON-ERC - HORIZON ERC Grants

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(opens in new window) ERC-2024-STG

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Host institution

HELSINGIN YLIOPISTO
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 2 175 054,00
Address
FABIANINKATU 33
00014 HELSINGIN YLIOPISTO
Finland

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Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 2 175 054,00

Beneficiaries (1)

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