Project description
Advanced NMR spectroscopy for studying large protein complexes
Nuclear magnetic resonance (NMR) spectroscopy is an analytical technique that reveals the atoms and how they interact within molecules. Researchers rely on NMR to examine protein structure and understand their function in health and disease. However, studying large protein complexes remains challenging due to signal overlap and intricate sample preparation. With the support of the Marie Skłodowska-Curie Actions programme, the PINPOINT-NMR project introduces an innovative method for labelling specific sites within large proteins. This targeted approach promises to simplify NMR spectra and accelerate analysis. As a result, it will make it easier to study large biomolecules such as therapeutic antibodies, supporting biomedical research and drug development.
Objective
The cell is an ensemble of dynamic molecular machines. NMR spectroscopy is the method of choice to observe complex conformational changes, transient interactions, and dynamics of proteins at atomic resolution. The introduction of methyl-specific labeling has made it possible to study protein assemblies of several hundred kDa by solution NMR. However, the high number of overlapping signals and the labor-intensive process of site specific analysis hamper NMR investigation of large hetero-oligomeric assemblies at room temperature. While segmental labeling or site-specific labeling offer opportunities to simplify 2D NMR spectra, the complexity of sample preparation or the characteristic low expression yield drastically limit its subsequent applications. Therefore, efficient, widely applicable, and straightforward labeling strategies are necessary to integrate solution NMR analysis of large proteins (such as monoclonal antibodies) into R&D pipelines for biomedical industry. I propose to harness the natural (anti)codon code for multi-site, sequence-specific isotope labeling. The resulting method would simplify the spectral congestion typical of 2D NMR spectra of large proteins, allowing targeted investigation of sites of interest (e.g. catalytic centers) and streamlining residue assignment. This method will reduce the time required for residue specific analysis of NMR data from months to days, thus expanding our ability to study large, medically-relevant complexes and address current biological challenges.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences physical sciences optics spectroscopy
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA)
MAIN PROGRAMME
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Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) HORIZON-MSCA-2024-PF-01
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
38058 GRENOBLE
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.