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Defining the role of stromal cells in determining HCC patient response to immunotherapies at single-cell resolution

Project description

Precision oncology for hepatocellular carcinoma

Liver cancer remains a major global health concern. There is limited understanding of the populations at risk of developing the disease, as well as treatment resistance and a lack of personalised therapeutic options. With the support of the Marie Skłodowska-Curie Actions programme, the STRIM-HCC project aims to uncover the mechanisms driving the progression of hepatocellular carcinoma (HCC) and the resistance to current immune-based therapies by integrating single-cell, genomic and transcriptomic tumour data using advanced bioinformatics. Special emphasis will be given on cancer-associated fibroblasts that influence therapy response. By connecting these insights to patient treatment outcomes, the project seeks to identify biomarkers of response to immune-checkpoint inhibitors and potential novel therapeutic targets, ultimately aiming to improve patient stratification and develop more effective treatments.

Objective

Hepatocellular carcinoma (HCC), remains a critical global health challenge, with limited treatment efficacy, particularly in advanced stages. The rising incidence of HCC is intricately linked to metabolic dysfunctions, such as metabolic-associated fatty liver disease (MAFLD) and its severe form, metabolic-associated steatohepatitis (MASH), highlighting an urgent need for enhanced precision oncology approaches. Despite recent advances, the lack of effective biomarkers and precision therapies for HCC underscores the complexity and heterogeneity of the disease.

This proposal aims to address these gaps by integrating cutting-edge bioinformatics tools and genomic data to uncover novel mechanisms of HCC progression and treatment resistance. We will focus on three primary objectives: (1) Characterizing the immunosuppressive tumor microenvironment (TME) in HCC, emphasizing cell-type-specific regulatory programs and enhancer-promoter interactions that influence tumor progression and immune evasion. (2) Identifying specific cancer-associated fibroblast (CAF) subtypes and their role in patient response to immune-based combination therapies, particularly atezolizumab and bevacizumab. (3) Leveraging whole-genome sequencing (WGS) to pinpoint targetable mutations in regulatory regions, which can guide personalized treatment strategies and improve patient outcomes.

By analyzing single-cell data and integrating it with whole-genome and RNA sequencing, this project aims to develop a comprehensive understanding of HCC's molecular underpinnings and its interaction with the TME. The expected outcomes include identifying new biomarkers for patient stratification, elucidating the role of CAFs in therapy response, and proposing actionable genomic targets for precision treatment. This research promises to revolutionize HCC management by providing innovative strategies for better-targeted therapies and improving clinical outcomes through enhanced precision oncology methodologies.

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HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships

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Call for proposal

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(opens in new window) HORIZON-MSCA-2024-PF-01

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Coordinator

FUNDACIO DE RECERCA CLINIC BARCELONA-INSTITUT D INVESTIGACIONS BIOMEDIQUES AUGUST PI I SUNYER
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 194 074,56
Address
CARRER ROSSELLO 149
08036 BARCELONA
Spain

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Region
Este Cataluña Barcelona
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Total cost

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