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DeClocking the vascular rhythmic control of tissue homeostasis and disease initiation

Project description

Circadian regulation of vascular homeostasis

Blood vessel-lining endothelial cells (ECs) form a dynamic interface between the circulation and tissues. ECs are therefore endowed with the critical task of sensing changes in the circulation and relaying them to parenchymal cells, acting as gatekeepers of homeostatic tissue function. Yet, the mechanisms that allow ECs to sense, adapt and rapidly respond to daily changes in the environment, such as light-dark cycles, feeding, or blood pressure fluctuations, remain unknown. The ERC-funded Vascular-Rhythm project will dissect how circadian changes regulate EC function and drive adaptive vascular responses. Researchers will employ multi-omics and computational analyses to uncover genetic programmes driving vascular homeostasis. Project results will provide fundamental insights into vascular biology and identify therapeutic targets for pathologies affected by circadian misalignment.

Objective

A quiescent, resilient layer of blood vessel-lining endothelial cells (ECs) is vital for maintaining organ function and enabling healthy aging. Much has been learnt in recent years about the mechanisms controlling the resilient EC phenotype. In turn, little is known if and how mechanisms of vascular resilience are affected by environmental rhythmic stimuli (extrinsic (e.g. light-dark, feeding-fasting) or intrinsic (e.g. blood pressure, hematopoiesis)). Performing day/night analyses of mouse liver and lung ECs, we identified distinct organ-specific time-of-the-day dependent transcriptomic programs of resting ECs. These translated functionally into temporal vascular adaptations during the day that critically sustain steady-state tissue health and function. Building on these stage-setting findings, the Vascular-Rhythm project will (i) analyze how ECs sense, adapt, and respond to circadian changes by uncovering rhythmic programs in healthy liver and lung ECs at mRNA, protein, and phospho-protein levels, and (ii) examine how the manipulation of vascular rhythms impacts physiological organ function, organismic aging, and disease initiation. To this end, we will implement a cross-species analytical platform (i.e. comparing nocturnal mice and diurnal pigs), integrate multiomics data with state-of-the-art computational methods, and perform in vitro & in vivo manipulatory assays to discover dynamic gene programs that underlie the homeostatic vascular endothelium and its adaptation to pathologic challenges. Collectively, adding the day/night cycle as a critical, hitherto in the field of vascular research heavily underappreciated research dimension, the Vascular-Rhythm project will fundamentally change the way we study and mechanistically understand blood vessel function. Moreover, the expected groundbreaking discoveries of the project have great potential to identify and validate novel therapeutic vascular vulnerabilities for multiple, circadian rhythm-affected pathologies.

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Topic(s)

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HORIZON-ERC - HORIZON ERC Grants

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Call for proposal

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(opens in new window) ERC-2025-STG

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Host institution

RUPRECHT-KARLS-UNIVERSITAET HEIDELBERG
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 1 499 705,00
Address
SEMINARSTRASSE 2
69117 Heidelberg
Germany

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Region
Baden-Württemberg Karlsruhe Heidelberg, Stadtkreis
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 1 499 705,00

Beneficiaries (1)

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