Environmental Nanoplastics Liver Impact and Variability in Endogenous Networks

Descripción del proyecto

Plásticos diminutos, grandes riesgos

Los microplásticos se han infiltrado en todos los ámbitos de nuestra vida diaria, incluyendo el aire que respiramos y los alimentos que comemos. El riesgo aumenta cuando se descomponen en nanoplásticos, ya que estas partículas más pequeñas pueden penetrar en las células y los órganos. Esto representa una amenaza tanto para el medio ambiente como para la salud humana, pero muy pocos estudios han evaluado los efectos a largo plazo. En este contexto, el equipo del proyecto financiado con fondos europeos ENLIVEN empleará organoides hepáticos humanos y embriones de pez cebra. para estudiar efectos de los nanoplásticos ambientales en la resistencia hepática y la respuesta génica y, de este modo, concienciar a la comunidad científica sobre los avances en nanotoxicología. El equipo del proyecto delineará las vías toxicológicas, mejorará la predicción de riesgos para la salud y aumentará las capacidades a largo plazo en materia de salud ambiental gracias a la secuenciación de ARN de célula única y metodologías punteras.

Objetivo

ENLIVEN is a research-through-training project designed to equip researcher with key expertise to tackle challenges in nanotoxicology. Plastic pollution has rapidly surpassed unprecedented in less than a century, with microplastics (MPs) found across various environments, ecosystems, and within the human body. MPs are present in the food we eat, the air we breathe, and the water we drink. While current analytical techniques limit the detection of nanoplastics (NPs <100 nm), plastic degradation continues beyond the microscale, leading to nanoscale interactions with potentially unknown consequences. NPs pose significant environmental and public health risks, particularly due to chronic exposure to harmful chemicals. Environmental nanoplastics (eNPs) are of particular concern due to their ability to penetrate cells and vital organs, increasing their retention in organisms. However, most research focuses on the environmental impact of MPs and NPs, leaving critical gaps in understanding their long-term effects on public health and biology. ENLIVEN aims to address this by uncovering the biological mechanisms of eNPs, examining their degradation, liver resilience, and long-term health impacts. Using human liver organoids and zebrafish embryos, ENLIVEN will study genes related to tolerance, sensitivity, and heritability, alongside liver resilience. The project proposes an innovative, integrative approach in predictive toxicology, combining bottom-up and top-down methodologies. It will link Adverse Outcome Pathways (AOP) through single-cell combinatorial RNA-seq and explore phenotypic variability via Drug-Induced Liver Injury studies in human liver organoids. This research will advance comparative human toxicology by revealing the biological mechanisms that respond to eNPs throughout life. Additionally, ENLIVEN will enhance researchers’ career prospects, foster international collaborations, and support the establishment of a research group at the home institution in Spain.