Gene-activated tri-layer 3D-bioprinted scaffold for enhanced osteochondral defect repair

Description du projet

Des implants à activation génique pour les articulations endommagées

Les lésions du cartilage et de l’os sous-jacent peuvent entraîner des douleurs chroniques, une instabilité articulaire et, à terme, de l’osthéoarthrite. Cependant, les traitements existants ne permettent que rarement de restaurer complètement les tissus. Soutenu par le programme Actions Marie Skłodowska-Curie, le projet GATSO-3D entend mettre au point un implant imprimé en 3D qui reproduit fidèlement la structure d’une articulation saine. Cet implant sera composé de trois couches libérant différents gènes thérapeutiques destinés à favoriser la régénération du cartilage et de l’os et à réduire l’inflammation. En combinant des biomatériaux de pointe, la nanotechnologie et la thérapie génique, cet implant vise à stimuler une réparation tissulaire plus efficace et durable. Cette approche innovante pourrait réduire le risque d’osthéoarthrite et améliorer la récupération après des lésions articulaires ainsi que la qualité de vie de millions de patients.

Objectif

Osteochondral defects (OCDs) arise from trauma, inflammation, or degenerative disease, causing pain, joint instability, and, in severe cases, osteoarthritis (OA). Current treatments are limited by the tissue’s hierarchical structure, poor cartilage repair capacity, and inflammation-driven degeneration, underscoring the need for advanced therapeutic approaches. The primary goal of this project is to construct a tri-layer 3D-bioprinted scaffold with structural, mechanical, and functional gradients that mimic native cartilage, calcified cartilage, and subchondral bone. The scaffold will enable spatial delivery of nanoparticles carrying osteogenic, chondrogenic, and anti-inflammatory plasmid DNA encodings (e.g. SOX9, BMP-2) from distinct layers, allowing evaluation of spatially programmed gene delivery in enhancing OCD repair. Prof. Fergal O’Brien’s group at the Royal College of Surgeons in Ireland has expertise in natural polymer-based scaffolds for nucleic acid delivery. I will combine my experience in advanced polymer synthesis and 3D-printed scaffold fabrication with their nanomedicine technology to develop gene-activated nanoparticle-integrated tri-layered 3D-printed scaffold, creating a localized gene delivery platform to enhance therapeutic efficacy and ultimately alleviate patient suffering. I will be supervised by Prof. O’Brien, and he will guide my Career Development Plan. I will develop complementary skills in project management, leadership, entrepreneurship, commercialization, dissemination, and profile enhancement. Acquiring advanced research competencies and complementary skills, I will mature as an independent scientist, enhancing my professional visibility, capacity, and competitiveness in the field of biomaterials and tissue engineering. This Fellowship will significantly contribute to the achievement of my long-term career goal, which is to become an independent scientist leading my own research group focused on novel scaffolds for tissue engineering.