Gene-activated tri-layer 3D-bioprinted scaffold for enhanced osteochondral defect repair

Descrizione del progetto

Impianti attivati geneticamente per le articolazioni danneggiate

I danni alla cartilagine e all’osso sottostante possono causare dolore cronico, instabilità articolare e, nel tempo, osteoartrosi. Tuttavia i trattamenti attualmente disponibili raramente consentono una completa rigenerazione dei tessuti. Con il sostegno del programma di azioni Marie Skłodowska-Curie, il progetto GATSO-3D svilupperà un impianto stampato in 3D che riproduce fedelmente la struttura di un’articolazione sana. L’impianto sarà costituito da tre strati che rilasciano diversi geni terapeutici progettati per favorire la rigenerazione della cartilagine e dell’osso e ridurre l’infiammazione. Combinando biomateriali avanzati, nanotecnologie e terapia genica, l’impianto mira a stimolare una riparazione dei tessuti più efficace e duratura. Tale approccio innovativo potrebbe migliorare il recupero in seguito a lesioni articolari, ridurre il rischio di osteoartrosi e migliorare la qualità della vita di milioni di pazienti.

Obiettivo

Osteochondral defects (OCDs) arise from trauma, inflammation, or degenerative disease, causing pain, joint instability, and, in severe cases, osteoarthritis (OA). Current treatments are limited by the tissue’s hierarchical structure, poor cartilage repair capacity, and inflammation-driven degeneration, underscoring the need for advanced therapeutic approaches. The primary goal of this project is to construct a tri-layer 3D-bioprinted scaffold with structural, mechanical, and functional gradients that mimic native cartilage, calcified cartilage, and subchondral bone. The scaffold will enable spatial delivery of nanoparticles carrying osteogenic, chondrogenic, and anti-inflammatory plasmid DNA encodings (e.g. SOX9, BMP-2) from distinct layers, allowing evaluation of spatially programmed gene delivery in enhancing OCD repair. Prof. Fergal O’Brien’s group at the Royal College of Surgeons in Ireland has expertise in natural polymer-based scaffolds for nucleic acid delivery. I will combine my experience in advanced polymer synthesis and 3D-printed scaffold fabrication with their nanomedicine technology to develop gene-activated nanoparticle-integrated tri-layered 3D-printed scaffold, creating a localized gene delivery platform to enhance therapeutic efficacy and ultimately alleviate patient suffering. I will be supervised by Prof. O’Brien, and he will guide my Career Development Plan. I will develop complementary skills in project management, leadership, entrepreneurship, commercialization, dissemination, and profile enhancement. Acquiring advanced research competencies and complementary skills, I will mature as an independent scientist, enhancing my professional visibility, capacity, and competitiveness in the field of biomaterials and tissue engineering. This Fellowship will significantly contribute to the achievement of my long-term career goal, which is to become an independent scientist leading my own research group focused on novel scaffolds for tissue engineering.