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The use of Methylated DNA Immunoprecipitation MeDIP in cancer for better clinical management

Objective

Aberrant DNA methylation is the most common molecular lesion of the cancer cell. Neither gene mutation nor cytogenetic abnormalities are as common in human tumours as DNA methylation alterations. The stability of our genome and correct gene expression is maintained to a great extent thanks to a perfectly preestablished pattern of DNA methylation and histone modifications. In cancer this idealistic scenario breaks down due to an interesting phenomenon whereby the regulatory regions (CpG islands) of certain tumour suppressor genes become hypermethylated, inactivating the gene as a consequence, whilst a wave of hypomethylation occurs in the genome. Indeed, CpG island promoter hypermethylation has a tumour-type-specific pattern, where each gene tends to be methylated in the cancer cells driven from a particular tissue, but not from others. It is also widely accepted that the multiprotein complex associated with the methylated DNA is also crucially involved in the repression of gene expression. Another aspect crucial for the interest of epigenetics in cancer is that promoter hypermethylation of the CpG island of tumours suppressor genes occurs early in tumorigenesis. Furthermore, it has been extensively reported that CpG promoter hypermethylation can be used as predictor of cancer behaviour and a predictor of response to treatment. This characteristic makes promoter hypermethylation combined with the epigenetic associated proteins profile, a potential biomarker for early detection of cancer and for the individualization of cancer treatment. We are going to use a novel technique based in chromatin immunoprecipitation, the Methylated DNA Immunoprecipitation (MeDIP) technique that will easily permit us to obtain an epigenomic profile which will be used to personalize cancer treatment and facilitate tumour diagnosis, prognosis and monitoring.

Field of science

  • /natural sciences/biological sciences/genetics and heredity/genome
  • /natural sciences/biological sciences/genetics and heredity/dna
  • /natural sciences/biological sciences/genetics and heredity/epigenetics
  • /medical and health sciences/clinical medicine/cancer

Call for proposal

FP7-HEALTH-2007-A
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Funding Scheme

CP-FP - Small or medium-scale focused research project

Coordinator

FUNDACIO INSTITUT D'INVESTIGACIO BIOMEDICA DE BELLVITGE
Address
Avenida Gran Via Hospitalet 199-203
08908 L'hospitalet De Llobregat
Spain
Activity type
Research Organisations
EU contribution
€ 539 343,31
Administrative Contact
Vistoria Cochrane (Ms.)

Participants (6)

UNIVERSITE LIBRE DE BRUXELLES
Belgium
EU contribution
€ 649 999
Address
Avenue Franklin Roosevelt 50
1050 Bruxelles
Activity type
Higher or Secondary Education Establishments
Administrative Contact
François Fuks (Dr.)
STICHTING KATHOLIEKE UNIVERSITEIT
Netherlands
EU contribution
€ 659 498
Address
Geert Grooteplein Noord 9
6525 EZ Nijmegen
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Ivo Hauer (Mr.)
UNIVERSITA DEGLI STUDI DELLA CAMPANIA LUIGI VANVITELLI
Italy
EU contribution
€ 299 998
Address
Viale Abramo Lincoln 5
81100 Caserta
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Annunziata Valente (Dr.)
Klinik Max Planck Institut für Psychiatrie
Germany
EU contribution
€ 299 999
Address
Hofgartenstrasse 8
80539 Muenchen
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Volker Geiss (Mr.)
DIAGENODE
Belgium
EU contribution
€ 265 499
Address
Rue Du Bois Saint Jean 3 Liege Park Science
4102 Seraing
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Didier Allaer (Mr.)
FUNDACION CENTRO NACIONAL DE INVESTIGACIONES ONCOLOGICAS CARLOS III

Participation ended

Spain
EU contribution
€ 285 657,69
Address
Calle Melchor Fernandez Almagro 3
28029 Madrid
Activity type
Research Organisations
Administrative Contact
Juan José Collazo Nieto (Mr.)