Objetivo Although the definition of latency under a clinical point of view seems clear, the bacterial biology behind that clinical situation remains poorly understood. While dormant, the tubercle bacilli are considered to be under non-replicating (NR) stage. In such a condition, bacilli are not only difficult to be detected but also refractory to the standard treatments avoiding their clearance from the infected tissues. The proposal has been built with the intend of providing tools to understand the bacterial mechanisms that leads to metabolic stage of M. tuberculosis during dormancy as the basis of sorting out the detection and treatment of latent infection. Several models of analysis have been developed trying to characterize dormant tubercle bacilli. Those models are ranging from in vitro conditions, such as hypoxia or starvation, to in vivo analysis, such as the animal model. We propose not only to study a complete range of those previously tested conditions, but also checking some other putative newly described, such as the recently described adipocytes ex vivo model, the new developments of the classical model of hypoxia, and the use of guinea-pigs as more adequate animal model of latent infection. Moreover, a set of drug combinations will be applied to determine their capability of clearance of the bacterial load. Due to its central role in the bacterial metabolism and growth we will analyze the non-replicating stage of the M. tuberculosis bacilli by determining the pre-rRNA synthesis. Finally, the cellular and tissue distribution of dormant bacilli will be also tested during in vivo latent infection both in the animal model and in clinical human samples. Research on the metabolic conditions of the dormant bacilli inside hosts, will provide important and invaluable insight into the biology of M. tuberculosis. That knowledge may lead to the development of novel strategies targeted at the control of the latent infection. Ámbito científico natural sciencesbiological sciencesmicrobiologybacteriologymedical and health sciencesclinical medicinepneumologytuberculosis Palabras clave Dormancy models Latent Tuberculosis Treatment of latent TB Programa(s) FP7-HEALTH - Specific Programme "Cooperation": Health Tema(s) HEALTH-2007-2.3.2-2 - Highly innovative approaches for research into host-pathogen interaction in tuberculosis HEALTH-2007-2.3.2-3 - Development of fast tests for the diagnosis of Multi-Drug-Resistant strains of HIV, malaria and tuberculosis and of latent tuberculosis infection (LTBI) Convocatoria de propuestas FP7-HEALTH-2007-A Consulte otros proyectos de esta convocatoria Régimen de financiación CP-FP - Small or medium-scale focused research project Coordinador UNIVERSIDAD AUTONOMA DE MADRID Aportación de la UE € 483 440,00 Dirección CALLE EINSTEIN 3 CIUDAD UNIV CANTOBLANCO RECTORADO 28049 Madrid España Ver en el mapa Región Comunidad de Madrid Comunidad de Madrid Madrid Tipo de actividad Higher or Secondary Education Establishments Contacto administrativo Ana Amigo (Ms.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos Participantes (6) Ordenar alfabéticamente Ordenar por aportación de la UE Ampliar todo Contraer todo ISTITUTO SUPERIORE DI SANITA Italia Aportación de la UE € 468 299,50 Dirección Viale Regina Elena 299 00161 Roma Ver en el mapa Región Centro (IT) Lazio Roma Tipo de actividad Research Organisations Contacto administrativo Rosa Maria Martoccia (Dr.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos INSTITUT DE INVESTIGACIO EN CIENCIES DE LA SALUT GERMANS TRIAS I PUJOL España Aportación de la UE € 310 160,00 Dirección CTRA DE CANYET S/N 08916 Badalona Barcelona Ver en el mapa Región Este Cataluña Barcelona Tipo de actividad Research Organisations Contacto administrativo Cristina Vilaplana Massaguer (Dr.) Enlaces Contactar con la organización Opens in new window Coste total Sin datos ST GEORGE'S HOSPITAL MEDICAL SCHOOL Reino Unido Aportación de la UE € 389 384,00 Dirección CRANMER TERRACE SW17 0RE London Ver en el mapa Región London Inner London — West Wandsworth Tipo de actividad Higher or Secondary Education Establishments Contacto administrativo Susan Mcpheat (Ms.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos INSTITUTO POLITECNICO NACIONAL México Aportación de la UE € 304 320,00 Dirección MIGUEL OTHON DE MENDIZABAL SN, COL. 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