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Ultra-high resolution and ultra-sensitive fluorescence methods for objective sub-cellular diagnosis of early disease and disease progression in breast and prostate cancer


Quenching of Triplet State Fluorophores for Studying Diffusion-Mediated Reactions in Lipid Membranes

Auteurs: Jerker Widengren; Lena Mäler; Sofia Johansson; Andriy Chmyrov; Johan Strömqvist; August Andersson
Identifiant permanent: PubMed ID:21112307; Digital Object Identifier:10.1016/j.bpj.2010.09.059; PubMed Central ID:PMC2998600

Note: a 4 ns hardware photon correlator based on a general-purpose field-programmable gate array development board implemented in a compact setup for fluorescence correlation spectroscopy.

Auteurs: Ralf Kühnemuth; Hayk Vardanyan; Claus A.M. Seidel; Stanislav Kalinin
Publié dans: AMER INST PHYSICS Review of Scientific Instruments; Vol 83 2012
Identifiant permanent: Digital Object Identifier:10.1063/1.4753994

Förster resonance energy transfer beyond 10 nm: exploiting the triplet state kinetics of organic fluorophores.

Auteurs: Thiemo Spielmann; Andriy Chmyrov; Jerker Widengren; Heike Hevekerl
Publié dans: AMER CHEMICAL SOC The Journal of Physical Chemistry B; Vol 115 2011
Identifiant permanent: Digital Object Identifier:10.1021/jp206770s

A compact STED microscope providing 3D nanoscale resolution.

Auteurs: Dominik Wildanger; Stefan W. Hell; Rebecca Medda; Lars Kastrup
Identifiant permanent: Digital Object Identifier:10.1111/j.1365-2818.2009.03188.x; PubMed ID:19772534

Filtered FCS: species auto- and cross-correlation functions highlight binding and dynamics in biomolecules.

Auteurs: Stanislav Kalinin; Hugo Sanabria; Claus A.M. Seidel; Suren Felekyan; Alessandro Valeri
Publié dans: WILEY-VCH Verlag ChemPhysChem; Vol 13 2012
Identifiant permanent: Digital Object Identifier:10.1002/cphc.201100897; PubMed Central ID:PMC3495305; PubMed ID:22407544

A new semi-automated instrument to improve the fine needle aspiration procedure during breast lesion cell sampling.

Auteurs: Hans Wiksell; Vilhelm Ekstrand; C. Wadström; G. Auer
Publié dans: Elsevier BV Physica Medica; Vol 25 2009
Identifiant permanent: Digital Object Identifier:10.1016/j.ejmp.2008.09.001

Modulation filtering enables removal of spikes in fluorescence correlation spectroscopy measurements without affecting the temporal information.

Auteurs: Per Thyberg; Jerker Widengren; Gustav Persson; Tor Sandén
Publié dans: AMER CHEMICAL SOC 2009
Identifiant permanent: Digital Object Identifier:10.1021/jp902538b

A STED microscope aligned by design

Auteurs: Stefan W. Hell; Lars Kastrup; Johanna Bückers; Dominik Wildanger; Volker Westphal
Publié dans: OPTICAL SOC AMER 2009
Identifiant permanent: Digital Object Identifier:10.1364/oe.17.016100

Iodide as a Triplet State Promoter and Quencher –Mechanisms and Possible Implications

Auteurs: Chmyrov, Andriy; Sandén, Tor; Widengren, Jerker
Publié dans: KTH, Experimentell biomolekylär fysik Array

Spatial distribution of Na + -K + -ATPase in dendritic spines dissected by nanoscale superresolution STED microscopy

Auteurs: Jerker Widengren; Hans Blom; Hans Blom; Anita Aperia; Daniel Rönnlund; Hjalmar Brismar; Hjalmar Brismar; Hjalmar Brismar; Alexander A. Bondar; Zuzana Spicarova; Lena Scott
Publié dans: Springer Nature BMC Neuroscience, Vol 12, Iss 1, p 16 (2011) 2011
Identifiant permanent: Digital Object Identifier:10.1186/1471-2202-12-16; PubMed Central ID:PMC3040715; PubMed ID:21272290

Recovery of photoinduced reversible dark States utilized for molecular diffusion measurements

Auteurs: Andriy Chmyrov; Tor Sandén; Jerker Widengren
Identifiant permanent: Digital Object Identifier:10.1021/ac1014047.s001; Digital Object Identifier:10.1021/ac1014047

Fluorescence-based transient state monitoring for biomolecular spectroscopy and imaging.

Auteurs: Jerker Widengren
Publié dans: The Royal Society 2010
Identifiant permanent: PubMed Central ID:PMC2894879; Digital Object Identifier:10.1098/rsif.2010.0146; PubMed ID:20375039

Iodide as a fluorescence quencher and promoter--mechanisms and possible implications.

Auteurs: Andriy Chmyrov; Jerker Widengren; Tor Sandén
Publié dans: KTH, Experimentell biomolekylär fysik 2010
Identifiant permanent: Digital Object Identifier:10.1021/jp103837f

DNA Damage Recognition via Activated ATM and p53 Pathway in Nonproliferating Human Prostate Tissue

Auteurs: Taija af Hällström; Hannu Koistinen; Ville Rantanen; Sari Jäämaa; Angelo M. De Marzo; Zhewei Zhang; Zhiming Yang; Kimmo Taari; Anna Sankila; Marikki Laiho; Mirja Ruutu; Leif C. Andersson; Leif C. Andersson; Ulf-Håkan Stenman
Publié dans: American Association for Cancer Research (AACR) 2010
Identifiant permanent: Digital Object Identifier:10.1158/0008-5472.can-10-0937

Förster Resonance Energy Transfer - from single molecule spectroscopy to imaging

Auteurs: Sisamakis, Evangelos
Publié dans: KTH, Experimentell biomolekylär fysik Array 2012

Nearest neighbor analysis of dopamine D1 receptors and Na+‐K+‐ATPases in dendritic spines dissected by STED microscopy

Auteurs: Hans Blom; Hans Blom; Anita Aperia; Hjalmar Brismar; Hjalmar Brismar; Hjalmar Brismar; Lena Scott; Ville Rantanen; Jerker Widengren; Zuzana Spicarova; Daniel Rönnlund
Identifiant permanent: Digital Object Identifier:10.1002/jemt.21046

Multiparameter fluorescence image spectroscopy to study molecular interactions.

Auteurs: Wolfgang Becker; Andrea Bleckmann; Rüdiger Simon; Ralf Kühnemuth; Stefanie Weidtkamp-Peters; Suren Felekyan; Claus A.M. Seidel
Publié dans: Royal Soc Chem Photochemical & Photobiological Sciences; Vol 8 2009
Identifiant permanent: Digital Object Identifier:10.1039/b903245m

Anima: Modular Workflow System for Comprehensive Image Data Analysis

Auteurs: Ville Rantanen; Sampsa Hautaniemi; Miko Valori
Publié dans: Frontiers Media S.A. 2014
Identifiant permanent: Digital Object Identifier:10.3389/fbioe.2014.00025; PubMed Central ID:PMC4115631; PubMed ID:25126541

Simultaneous multi-lifetime multi-color STED imaging for colocalization analyses.

Auteurs: Lars Kastrup; Dominik Wildanger; Giuseppe Vicidomini; Johanna Bückers; Stefan W. Hell
Identifiant permanent: Digital Object Identifier:10.1364/oe.19.003130

Trans-cis isomerization of lipophilic dyes probing membrane microviscosity in biological membranes and in live cells.

Auteurs: Heike Hevekerl; Volodymyr Chmyrov; Thiemo Spielmann; Jerker Widengren
Publié dans: KTH, Experimentell biomolekylär fysik 2015
Identifiant permanent: Digital Object Identifier:10.1021/acs.analchem.5b00863

Prevention of tumour cell dissemination in diagnostic needle procedures

Auteurs: Hans Wiksell; Sandberg Po; Karin Leifland; Janicijevic M; Lars Löfgren; Kai-Uwe Schässburger; Samuel Rotstein; C. Wadström; Gert Auer
Publié dans: Nature Publishing Group British Journal of Cancer 2010
Identifiant permanent: Digital Object Identifier:10.1038/sj.bjc.6605964; PubMed Central ID:PMC2994228; PubMed ID:21045831

FLIM and FCS detection in laser-scanning microscopes: increased efficiency by GaAsP hybrid detectors.

Auteurs: Oliver Holub; B. Su; Klaus Weisshart; Wolfgang Becker
Identifiant permanent: Digital Object Identifier:10.1002/jemt.20959

A new specially designed needle significantly increases sample yield during fine needle aspiration of breast lesions.

Auteurs: C. Wadström; Hans Wiksell; G. Auer; Vilhelm Ekstrand
Identifiant permanent: Digital Object Identifier:10.1016/j.ejmp.2008.01.009

Stimulated Emission Depletion Microscopy

Auteurs: Jerker Widengren; Hans Blom
Publié dans: American Chemical Society (ACS) 2017
Identifiant permanent: Digital Object Identifier:10.1021/acs.chemrev.6b00653

Axillary lymph node metastasis and survival in breast cancer patients with concurrent cardio-cerebral-vascular disease

Auteurs: Gert Auer; Kai-Uwe Schässburger; Nils Stormby; Roland Sennerstam; Hans Wiksell
Publié dans: Royal Soc of Medicine Press 2011
Identifiant permanent: Digital Object Identifier:10.1258/shorts.2010.010105; PubMed Central ID:PMC3046562; PubMed ID:21369530

Targeted chemotherapy for head and neck cancer with a chimeric oncolytic adenovirus coding for bifunctional suicide protein FCU1.

Auteurs: Tanja Hakkarainen; Johann Foloppe; Akseli Hemminki; Vincenzo Cerullo; Minna Eriksson; Anna Kanerva; Monika Lusky; Kilian Guse; Ville Rantanen; Sophie Escutenaire; Ilkka Liikanen; João D. Dias; Philippe Erbs; Iulia Diaconu; Sari Pesonen; Marta Sloniecka
Identifiant permanent: PubMed ID:20388844; Digital Object Identifier:10.1158/1078-0432.ccr-09-2974

DDR in Human Seminal Vesicle

Auteurs: Kimmo Taari; Leif C. Andersson; Anna Sankila; Mirja Ruutu; Ville Rantanen; Marikki Laiho; Marikki Laiho; Karita Peltonen; Päivi Järvinen; Taija af Hällström; Sari Jäämaa
Publié dans: WILEY-BLACKWELL The Prostate; Vol 72 2011
Identifiant permanent: Digital Object Identifier:10.1002/pros.21509