Objectif X-ray crystallography (XR) provides atomic resolution models of subunits of large biological assemblies, while electron microscopy (EM) allows lower-resolution imaging of whole macromolecular complexes. Combining the information from both techniques by fitting an atomic XR model into an EM density map enables in principle atomic-level interpretation of the EM data. We propose first to develop graphical software for manually fitting atomic XR models into EM data while simultaneously calculating the correlation between the models and the data using a fast reciprocal-space method [Navaza et al., Acta Crys. D58 (2002), 1820].Second, we plan to implement an automatic fitting procedure with a complete 6-dimensional (3 rotations and 3 translations) search, based on a recently developed 6D extension of the fast rotational matching technique [Kovacs et al., Acta Crys. D59 (2003), 1371]. As a complement to these methodological developments, we will study using EM the structure of SIRV1, a rudivirus infecting thermophilic Archaea [Prangishvili et al., Genetics 152 (1999), 1387]. We will then use our newly developed software to fit the structures of viral proteins into the EM reconstruction. Champ scientifique sciences naturellesinformatique et science de l'informationlogicielsciences naturellessciences biologiquesmicrobiologievirologiesciences naturellessciences de la Terre et sciences connexes de l'environnementgéologieminéralogiecristallographiesciences naturellessciences biologiquesbiochimiebiomoléculeprotéinessciences naturellessciences physiquesoptiquemicroscopieelectron microscopy Programme(s) FP6-MOBILITY - Human resources and Mobility in the specific programme for research, technological development and demonstration "Structuring the European Research Area" under the Sixth Framework Programme 2002-2006 Thème(s) MOBILITY-4.2 - Marie Curie International Reintegration Grants (IRG) Appel à propositions FP6-2004-MOBILITY-12 Voir d’autres projets de cet appel Régime de financement EIF - Marie Curie actions-Intra-European Fellowships Coordinateur CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE Adresse 3, rue michel ange Paris France Voir sur la carte Liens Site web Opens in new window Contribution de l’UE Aucune donnée
