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Massive Digital sequencing by binary DNA

Objective

DNA sequencing costs have fallen more than 50-fold over the past decade, driven part by tools, technology and process improvements made during the Human Genome Project. However, it still costs around $10 million to sequence 3 billion base pairs - the amount of DNA found in the genomes of humans and other mammals. 1st generation sequencing platforms, which are based upon capillary electrophoresis and fluorescence detection, have been around since 1992. Due to poor price/performance parameters several initiatives have been launched to facilitate a replacement of this sequencing technology. Most notably is the US National Institute of Health with the goal of developing 2nd and 3rd generation technologies that can read a human genome for $100.000 and $1.000 respectively. To date four vendors have introduced 2nd generation platforms to the market and they have the capacities of sequencing between 0,5 to 2 Gb per 24 hours. The main limitation for improved price/performance of 2nd generation platforms is that they are based on an expensive CCD-camera set up. As a consequence new and radical approaches are required to meet the $1.000 genome goal. This proposal aims to develop a high-throughput low cost 3rd generation DNA sequencing instrument, by deploying novel DNA preparation methods and read-out technologies. The potential instrument output is 200-300Gbp raw sequence per run, which allows for whole genome sequencing on a mass scale. A consortium of five European partners with multidisciplinary expertise has been established to reach the overall objective of this ambitious project.

Field of science

  • /social sciences/economics and business/business and management/commerce
  • /natural sciences/biological sciences/zoology/mammalogy
  • /natural sciences/chemical sciences/electrochemistry/electrophoresis
  • /natural sciences/biological sciences/genetics and heredity/genome

Call for proposal

FP7-HEALTH-2007-B
See other projects for this call

Funding Scheme

CP-TP - Collaborative Project targeted to a special group (such as SMEs)

Coordinator

KUNGLIGA TEKNISKA HOEGSKOLAN
Address
Brinellvagen 8
100 44 Stockholm
Sweden
Activity type
Higher or Secondary Education Establishments
EU contribution
€ 1 087 682,47
Administrative Contact
åke Eriksson (Mr.)

Participants (4)

LINGVITAE HOLDING AS

Participation ended

Norway
EU contribution
€ 1 049 720,23
Address
Drammensveien 165
0277 Oslo
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Preben Lexow (Dr.)
Plarion Ltd
United Kingdom
EU contribution
€ 368 707,61
Address
Beech House, Melbourn Science Park, Cambridge Road Unit B3
SG8 6HB Melbourn
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Robert James Longman (Mr.)
KAROLINSKA INSTITUTET
Sweden
EU contribution
€ 320 243,66
Address
Nobels Vag 5
17177 Stockholm
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Thomas Walstam (Mr.)
INSTITUT DE LA CORROSION SASU
France
EU contribution
€ 173 646,03
Address
Rue Rivoalon 220 Technopole De Brest Iroise
29200 Brest
Activity type
Research Organisations
Administrative Contact
Dominique Thierry (Dr.)