Entering a new era of DNA sequencing
After completion of the Human Genome project, where the entire human genome was sequenced, technical advancements significantly reduced the cost and time of DNA sequencing. Nonetheless, it remains an extremely expensive technique due to the instrument setup. The principle aim of the EU-funded 'Massive digital sequencing by binary DNA' (Digital Sequencing) project is to develop a technological platform capable of high-throughput sequencing. The consortium is basing their idea on the optical disk technology used in DVDs and PCs to generate the first 'Digital Sequencer'. A novel technology has been developed for sample preparation that converts DNA into enlarged synthetic molecules with digital features (0, 1) called 'binary tags'. These are then further formulated into long virtual DNA reads to match the bit size required for reading them on an optical disk reader. By developing a nanoprinting-based technique, laser induced transfer (LIT), the consortium has managed to transfer the DNA onto spots of nanometre size. An early laboratory prototype reader is subsequently used to obtain the binary format of the DNA sequences. Although DNA analysis is primarily used in research for drug development companies and diagnostics, this is expected to change once the cost comes down. Project partners believe that implementation of the 'Digital Sequencer' will extend the applications of DNA sequencing to healthcare, agriculture and forensic medicine.
