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Molecular Dissection of Melanoma Progression: An integrated Pan-European Approach

Objective

Transformed melanocytes use several strategies to enable their progression to advanced melanoma and metastasis, including genetic and epigenetic alterations, along with disruptions to gene and protein expression. This consortium aims to identify novel biomarkers for predicting the progression and prognosis of melanoma. To achieve this, a concerted collaborative effort is proposed here between key academic and industrial partners, centred on intersectorial training of experienced researchers. Our first goal is to identify novel methylated genes via several complementary screening methods, followed by validation extensive melanoma cohorts available to the consortium. We will use the following strategies to identify methylated genes: (1) an array-based technique called MeDIP-ON-CHIP, (2) a high-throughput bisulphite-based technique developed by Illumina (to simultaneously analyse up to 800 genes over 96 samples) and (3) in silico analysis of DNA microarray data. To validate these methylation targets, we will use both qualitative and quantitative methylation-specific PCR (MSP) and determine if these methylation events correlate with melanoma progression, i.e. from benign and atypical nevi to primary and metastatic tumours. Our second goal is to validate genes that associate with melanoma progression and prognosis. For this aspect of the project, we will concentrate on the application of tissue microarray technology and associated image analysis approaches to validate molecular determinants of melanoma progression. Prioritised targets will then be functionally validated by a unique in vitro assay that models extravasation, or exit of cancer cells out of the blood stream, a key step in the metastatic process. Overall, these studies will give clues to melanoma progression, and may identify important targets for melanoma treatment. In addition, this industry-academic partnership will provide a valuable cross-disciplinary training ground for emerging researchers.

Call for proposal

FP7-PEOPLE-IAPP-2008
See other projects for this call

Funding Scheme

MC-IAPP - Industry-Academia Partnerships and Pathways (IAPP)

Coordinator

UNIVERSITY COLLEGE DUBLIN, NATIONAL UNIVERSITY OF IRELAND, DUBLIN
Address
Belfield
4 Dublin
Ireland
Activity type
Higher or Secondary Education Establishments
EU contribution
€ 354 949
Administrative Contact
Donal Doolan (Mr.)

Participants (8)

UNIVERSITEIT MAASTRICHT
Netherlands
EU contribution
€ 67 157
Address
Minderbroedersberg 4
6200 MD Maastricht
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Sietske Satijn (Ms.)
KATHOLIEKE UNIVERSITEIT LEUVEN
Belgium
Address
Oude Markt 13
3000 Leuven
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Elke Lammertyn (Dr.)
UPPSALA UNIVERSITET
Sweden
EU contribution
€ 198 849
Address
Von Kraemers Alle 4
751 05 Uppsala
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Fredrik Ponten (Prof.)
ONCOMETHYLOME BVBA

Participation ended

Belgium
Address
Gaston Geenlaan 1
3001 Leuven
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Joost Louwagie (Mr.)
SlidePath

Participation ended

Ireland
Address
Invent Building, Dublin City University
9 Dublin
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Sinead Kilkelly (Ms.)
CELLIX LIMITED
Ireland
EU contribution
€ 198 994
Address
Unit 1, Longmile Business Park, Longmile Rd
D12 EK79 Dublin
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Vivienne Williams (Ms.)
ONCOMARK LIMITED
Ireland
EU contribution
€ 794 659
Address
172 Rathgar Road Rathgar
Dublin 6 Dublin
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Stephen Penney (Mr.)
FUNDACIO INSTITUT D'INVESTIGACIO BIOMEDICA DE BELLVITGE
Spain
EU contribution
€ 121 875
Address
Avenida Gran Via Hospitalet 199-203
08908 L'hospitalet De Llobregat
Activity type
Research Organisations
Administrative Contact
Victoria Cochrane (Ms.)