Objective
The purification, renewal and differentiation of native cardiac progenitors would form a mechanistic underpinning for unravelling steps for both cardiac lineage formation and regeneration, and their links to forms of congenital and adult cardiac diseases. Taking advantage of a developmental lineage marker for undifferentiated cardiogenic precursors as a requirement for a heart-specific origin, we have identified in the post-natal heart a novel cardiac cell type. The LIM-homeodomain transcription factor isle t-1 (isl1) marks a cell population that makes a substantial contribution to the embryonic heart. Tamoxifen-inducible Cre/lox technology enables selective marking of this progenitor cell population including its progeny, at a defined time, and purification to relative homogeneity. Co-culture studies with neonatal myocytes indicate that isl1+ cells display a highly efficient conversion to a mature cardiac phenotype. Our hypothesis is that isl1 is a unique marker to isolate a population of cardiac precursors f rom the intact heart that can differentiate in vitro into functioning myocytes and to generate cardiogenic precursors from mouse embryonic stem cell systems during cardiogenesis. Our specific aims are: 1) To determine therapeutic efficacy of isl1+ cardiobl asts by transplantation into normal and diseased myocardium utilizing mouse models. 2) To establish optimal conditions for generation and differentiation of isl1+ progenitors in the mouse embryonic stem cell system. 3) To identify specific molecular pathwa ys that drive the differentiation of isl1+ cardioblasts into specific cardiomyocyte cell lineages. (Laugwitz K-L, Moretti A, Lam J, Gruber P, Chen Y, Woodard S, Lin L, Cai C-L, Lu M, Reth M, Platoshyn O, Yuan J, Evans S & Chien KR. Postnatal isl1+ card ioblasts enter fully differentiated cardiomyocyte lineages. Nature 433: 647-653 (2005).
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences medical biotechnology cells technologies stem cells
- medical and health sciences basic medicine pathology
- medical and health sciences clinical medicine transplantation
- medical and health sciences clinical medicine embryology
You need to log in or register to use this function
Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2004-MOBILITY-8
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
MÜNCHEN
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.