Objectif Carbohydrate-rich diets are frequently used in the commercial farming of fish. However, fish do not appear to have an elevated capacity to clear a high load of glucose from their circulation. Special attention has been directed towards the study of the physiological responses to such carbohydrate-rich feeding. Skeletal muscles play a key role in glucose homeostasis in mammals, and exercise has a noticeable effect in lowering plasma glucose. This effect is caused by an up-regulation of glucose transport mediated by GLUT4 in skeletal muscles in response to exercise. However, the exact mediating mechanism in the modulation of glucose transport in skeletal muscle has not been entirely characterized in vertebrates. Moreover, fish are an excellent model for the research of mechanisms involved in changes of the GLUT glucose transporters produced by exercise because they have anatomically separated red and white muscle masses, which allow the measurement of fibre-type-specific responses. I hypothesize that sustained exercise can be used to enhance carbohydrate expenditure in fish, increasing their natural capacity to clear and utilize plasma glucose by the contracting muscle. I will investigate this possibility, placing a special emphasis on the study of the cellular and molecular mechanisms regulating the GLUT-1 and -4 glucose transporters in white and red muscles. Changes in patterns for gene expression using a microarray platform and key biochemical parameters will also be measured in red and white muscles of rainbow trout after four weeks of sustained swimming. I expect that the results obtained from this research will help us to understand how glucose is regulated in fish. This knowledge could be transferred to European companies, leading to more efficient and environmentally conscious feeding practices in the rapidly growing and economic important aquaculture sector. Champ scientifique agricultural sciencesagriculture, forestry, and fisheriesfisheriesnatural sciencesbiological sciencesbiochemistrybiomoleculescarbohydratesnatural sciencesbiological scienceszoologyichthyology Mots‑clés AMPK GLUT4 Natural sciences exercise fish insulin metabolic regulation muscle physiology swimming Programme(s) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Thème(s) FP7-PEOPLE-IIF-2008 - Marie Curie Action: "International Incoming Fellowships" Appel à propositions FP7-PEOPLE-IIF-2008 Voir d’autres projets de cet appel Régime de financement MC-IIF - International Incoming Fellowships (IIF) Coordinateur UNIVERSITAT DE BARCELONA Contribution de l’UE € 219 298,04 Adresse GRAN VIA DE LES CORTS CATALANES 585 08007 Barcelona Espagne Voir sur la carte Région Este Cataluña Barcelona Type d’activité Higher or Secondary Education Establishments Contact administratif Josep Planas (Dr.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée