Objective
AIM: To identify the molecular mechanisms characterizing cilium function, and the discrete perturbations associated with dysfunction caused by mutations in inherited ciliopathies, applying a systems biology approach. BACKGROUND: Cilia are microtubule-based, centriole-derived projections from the cell surface. They transduce extracellular signals and regulate key processes in which signals of the extracellular environment are translated into a cellular response, such as cell cycle control, Wnt signalling, Shh signalling and planar cell polarity. Disruption of cilium-based processes by mutations can cause very severe disorders. Many of these ciliopathies have overlapping phenotypes. There is evidence, that ciliary proteins are organized in cell/context specific complexes and/or in shared regulatory circuits in cilia of affected tissues. Yet, knowledge of the composition, wiring, dynamics and associated signaling pathways of the corresponding molecular building blocks and associated protein networks remains very limited. APPROACH: We propose here that ciliopathies can be considered systemically as specific perturbations in a versatile dynamically regulated multifunctional molecular machine. Mainly based on the comprehensive description of the ciliary interactome, quantitative functional assays as well as human genetic data derived from ciliopathy patients, we will generate a comprehensive stream of content-rich quantitative data towards systemic analysis of ciliar function. These data will be used to generate and validate discrete models that describe functional modules and regulatory circuits in the ciliome as well as predicting biological context specific features of cilia as well as perturbations leading to ciliopathies. This will enable us to 1) understand the systemic features of discrete ciliary functions, 2) scrutinize the molecular disease mechanisms of different overlapping ciliopathies, and 3) develop therapeutic strategies towards improved treatment.
Fields of science
Call for proposal
FP7-HEALTH-2009-two-stage
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Funding Scheme
CP-IP - Large-scale integrating projectCoordinator
6525 XZ Nijmegen
Netherlands
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Participants (16)
69117 Heidelberg
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27708 Durham Nc
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79106 Freiburg
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55122 Mainz
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00185 Roma
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3584 CX Utrecht
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WC1E 6BT LONDON
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LS2 9JT Leeds
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4 Dublin
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75654 Paris
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Participation ended
75794 Paris
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CB4 0WS Cambridge
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69117 Heidelberg
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48149 MUENSTER
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72074 Tuebingen
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91025 Evry
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