Objective
The rapidly decreasing costs of DNA sequencing have made the genomic sequences from thousands of pathogens broadly accessible. The utilization of this new knowledge in clinical practice, however, critically depends on the availability of new analytical tools and techniques that could quickly and efficiently detect the presence of specific genomic variants of pathogens. Some recent examples include the outbreak of H1N1 (Swine flu) and HIV-AIDS. Current approaches rely on costly and time-consuming Polymerase Chain Reaction (PCR), to achieve the specificity and quantity required by standard means of detection. Here I propose to develop a radically low-cost, single-molecule, genotyping method based on nanopore sensing of Peptide Nucleic Acids (PNA) markers. This method is designed to yield an extremely low cost, single-molecule detection of viral infections.
Nanopores are emerging single-molecule sensors, where an electrophoretic force threads DNA or RNA biopolymers, into a nanoscale aperture made in a thin film. The threading process uncoils the biopolymers, as they move from one side of the film to the other. Molecules entering the nanopore occlude some of the free ions in the solution from the pore volume, thus permitting real-time electrical detection of the local cross-section of the biopolymer. We propose to develop this method to permit the rapid detection of sequence-specific PNA markers, known to invade double-stranded DNA and form bulges at the points of invasion. We recently showed that PNAs can be detected using tiny solid-state nanopores. To transform this discovery into a robust analytical tool, extensive studies are now required to critically improve the nanopore fabrication, the signal over noise of the measurements, and the biomolecular strategies for efficient PNA invasion. Our studies will ultimately enable the development of low-cost, portable, and high-throughput devices for a broad range of genome based molecular diagnostics.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- natural sciences biological sciences biochemistry biomolecules nucleic acids
- natural sciences biological sciences genetics DNA
- medical and health sciences health sciences infectious diseases RNA viruses influenza
- natural sciences biological sciences genetics RNA
- natural sciences biological sciences genetics genomes
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2010-RG
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
32000 Haifa
Israel
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.