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Target binders

Objective

We want to further develop our tools and technologies for high-throughput research, with the final goals being (I.) the particle-based combinatorial synthesis of 1 Mio different peptides on a glass slide for chemical costs of ~50€ (KIT, CBL, MS, TUV), and (II.) the labelling-free parallel readout of binding affinities by a variant reflectometric interference spectroscopy method for ~10.000 peptide spots per cm(+2) when staining the array with an unlabeled protein (BIA, KIT). These tools provide the basis (III.) for a standardized, fast, and reliable high-throughput procedure that we want to develop in order to find high-affinity peptide binders against any pharmaceutically interesting target protein. Such a procedure might have an important impact in medicine and in the biotechnology industry. In order to achieve this goal, we will use display techniques that in combination with high-throughput sequencing typically will identify ~100.000 putative peptide binders per target protein (ISO). These will be synthesized in array format to validate binding to the target protein by an independent method (PPP, DKFZ). Next, based on binders from initial screens, many variant peptides are synthesized in high-density array format for iterative screens (PPP, DKFZ, KIT), whereby massive parallel labelling-free detection of binders pinpoints higher-affinity binders (BIA). In order to validate our novel high-throughput procedure, (IV.) we want to find high-affinity peptide binders against relevant target proteins (delivered by APO and OXF), and test these binders in biological assays (OXF, APO).

Call for proposal

FP7-HEALTH-2011-two-stage
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Coordinator

KARLSRUHER INSTITUT FUER TECHNOLOGIE
Address
Kaiserstrasse 12
76131 Karlsruhe
Germany
Activity type
Higher or Secondary Education Establishments
EU contribution
€ 609 050
Administrative Contact
Natascha Wallburg (Ms.)

Participants (9)

ISOGENICA LTD
United Kingdom
EU contribution
€ 700 500
Address
The Mansion, Chesterford Research Park
CB10 1XL Little Chesterford Saffron Walden
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Chris Ullman (Dr.)
Center for Advanced Bioanalysis GmbH
Austria
EU contribution
€ 549 000
Address
Scharitzerstrasse 6-8
4020 Linz
Activity type
Research Organisations
Administrative Contact
Alois Sonnleitner (Dr.)
PEPperPRINT GmbH
Germany
EU contribution
€ 459 000
Address
Schlossweg 9
69168 Wiesloch
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Volker Stadler (Dr.)
BIAMETRICS GMBH
Germany
EU contribution
€ 471 000
Address
Auf Der Morgenstelle 18
72076 Tubingen
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Guenther Proll (Dr.)
THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD
United Kingdom
EU contribution
€ 499 980
Address
Wellington Square University Offices
OX1 2JD Oxford
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Stephen Conway (Dr.)
Apogenix
Germany
EU contribution
€ 400 000
Address
Im Neuenheimer Feld 584
69120 Heidelberg
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Jürgen Gamer (Dr.)
DEUTSCHES KREBSFORSCHUNGSZENTRUM HEIDELBERG
Germany
EU contribution
€ 130 000
Address
Im Neuenheimer Feld 280
69120 Heidelberg
Activity type
Research Organisations
Administrative Contact
Bettina Crispin (Ms.)
MICROSYSTEMS LTD
Bulgaria
EU contribution
€ 100 000
Address
Chaika 15 D 21
9010 Varna
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Nikolay Nikolov (Mr.)
TECHNICAL UNIVERSITY OF VARNA
Bulgaria
EU contribution
€ 70 200
Address
Studentska 1
9010 Varna
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Jordan Kolev (Prof.)