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Standarization and orthogonalization of the gene expression flow for robust engineering of NTN (new-to-nature) biological properties

Objective

The ST-FLOW Project merges the efforts of 14 leading European research groups for developing material and computational standards that enable the forward-design of prokaryotic systems with a degree of robustness and predictability that is not possible with customary Genetic Engineering. The central issue at stake is the identification and implementation of rules that allow the conversion of given biological parts assembled with a set of principles for physical composition into perfectly predictable functional properties of the resulting devices, modules and entire systems. ST-FLOW focuses on each of the steps that go from assembling a DNA sequence encoding all necessary expression signals in a prokaryotic host (by default, E. coli) all the way to the making of the final product or to the behaviour of single cells and populations. Two complementary approaches will be adopted to solve the conundrum of physical composition vs. biological functionality of thereby engineered devices. In one case (bottom up), large combinatorial libraries of gene expression signals will be merged with suitable reporter systems and the input/output functions examined and parameterized in a high-throughput fashion. The expected outcome of this effort is to establish experience-based but still reliable rules and criteria for the assembly of new devices and systems -following the same physical composition rules or adopting CAD design. Yet, many outliers (combinations that do not follow the rules) are expected, and making sense of them will be the task of the complementary top-down approach. In this case, ST-FLOW will revisit some of gaps in our knowledge of the gene expression flow (transcription, mRNA fate, translation) that need to be addressed for engineering functional devices from first principles. Ethical, legal and societal issues will also be examined in a context of public dialogue and sound science communication.

Field of science

  • /medical and health sciences/medical biotechnology/genetic engineering

Call for proposal

FP7-KBBE-2011-5
See other projects for this call

Funding Scheme

CP-IP - Large-scale integrating project

Coordinator

AGENCIA ESTATAL CONSEJO SUPERIOR DEINVESTIGACIONES CIENTIFICAS
Address
Calle Serrano 117
28006 Madrid
Spain
Activity type
Research Organisations
EU contribution
€ 1 145 874
Administrative Contact
Alberto Sereno álvarez (Prof.)

Participants (14)

IMPERIAL COLLEGE OF SCIENCE TECHNOLOGY AND MEDICINE
United Kingdom
EU contribution
€ 992 248
Address
South Kensington Campus Exhibition Road
SW7 2AZ London
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Shaun Power (Mr.)
EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH
Switzerland
EU contribution
€ 982 722
Address
Raemistrasse 101
8092 Zuerich
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Sven Panke (Prof.)
THE UNIVERSITY OF BIRMINGHAM
United Kingdom
EU contribution
€ 383 922
Address
Edgbaston
B15 2TT Birmingham
Activity type
Higher or Secondary Education Establishments
Administrative Contact
May Chung (Ms.)
INSTITUTO DE TECNOLOGIA QUIMICA E BIOLOGICA - UNIVERSIDADE NOVA DE LISBOA
Portugal
EU contribution
€ 281 730
Address
Avenida Da Republica, Estacao Agronomica Nacional
2784-505 Oeiras
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Maria De Lurdes Conceicao (Dr.)
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS
France
EU contribution
€ 340 800
Address
Rue Michel Ange 3
75794 Paris
Activity type
Research Organisations
Administrative Contact
Adrien Nusca (Mr.)
LIFEWIZZ LDA

Participation ended

Portugal
Address
Rua Pero De Alenquer 123/7E
4150 616 Porto
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Vitor Martins Dos Santos (Prof.)
UNIVERSITE DE LAUSANNE
Switzerland
EU contribution
€ 335 520
Address
Quartier Unil-centre Bâtiment Unicentre
1015 Lausanne
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Jan Roelof Van Der Meer (Prof.)
BIOILIBERIS RESEARCH AND DEVELOPMENT SL
Spain
EU contribution
€ 205 200
Address
Av De La Innovacion 1 Parque Tecnologico De Ciencias Salud
18100 Armilla Granada
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Matilde Fernández (Dr.)
UNIVERSITAET STUTTGART
Germany
EU contribution
€ 307 584
Address
Keplerstrasse 7
70174 Stuttgart
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Ralf Takors (Prof.)
UNIVERSITAT DE VALENCIA
Spain
EU contribution
€ 341 315
Address
Avenida Blasco Ibanez 13
46010 Valencia
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Angeles Sanchis (Ms.)
BIOFACTION KG
Austria
EU contribution
€ 244 926
Address
Kundmanngasse 39/12
1030 Wien
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Markus Schmidt (Dr.)
TECHNISCHE UNIVERSITAET MUENCHEN
Germany
EU contribution
€ 67 200
Address
Arcisstrasse 21
80333 Muenchen
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Katrin Hörmann (Ms.)
THE UNIVERSITY OF EDINBURGH
United Kingdom
EU contribution
€ 87 969
Address
Old College, South Bridge
EH8 9YL Edinburgh
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Nisha Patel (Ms.)
Isthmus
France
EU contribution
€ 281 732
Address
Rue Saint Amand 31
75015 Paris
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Pierre Dedenys (Mr.)