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Standarization and orthogonalization of the gene expression flow for robust engineering of NTN (new-to-nature) biological properties

Objectif

The ST-FLOW Project merges the efforts of 14 leading European research groups for developing material and computational standards that enable the forward-design of prokaryotic systems with a degree of robustness and predictability that is not possible with customary Genetic Engineering. The central issue at stake is the identification and implementation of rules that allow the conversion of given biological parts assembled with a set of principles for physical composition into perfectly predictable functional properties of the resulting devices, modules and entire systems. ST-FLOW focuses on each of the steps that go from assembling a DNA sequence encoding all necessary expression signals in a prokaryotic host (by default, E. coli) all the way to the making of the final product or to the behaviour of single cells and populations. Two complementary approaches will be adopted to solve the conundrum of physical composition vs. biological functionality of thereby engineered devices. In one case (bottom up), large combinatorial libraries of gene expression signals will be merged with suitable reporter systems and the input/output functions examined and parameterized in a high-throughput fashion. The expected outcome of this effort is to establish experience-based but still reliable rules and criteria for the assembly of new devices and systems -following the same physical composition rules or adopting CAD design. Yet, many outliers (combinations that do not follow the rules) are expected, and making sense of them will be the task of the complementary top-down approach. In this case, ST-FLOW will revisit some of gaps in our knowledge of the gene expression flow (transcription, mRNA fate, translation) that need to be addressed for engineering functional devices from first principles. Ethical, legal and societal issues will also be examined in a context of public dialogue and sound science communication.

Champ scientifique

Programme(s)

Thème(s)

Appel à propositions

FP7-KBBE-2011-5
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Régime de financement

CP-IP - Large-scale integrating project

Coordinateur

AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
Contribution de l’UE
€ 1 145 874,00
Adresse
CALLE SERRANO 117
28006 Madrid
Espagne

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Région
Comunidad de Madrid Comunidad de Madrid Madrid
Type d’activité
Research Organisations
Contact administratif
Alberto Sereno álvarez (Prof.)
Liens
Coût total
Aucune donnée

Participants (14)

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Dernière mise à jour: 11 Decembre 2017

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Permalink: https://cordis.europa.eu/project/id/289326/fr

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