Microfluidic assay for rapid multiplexed detection of bacterial urinary tract infections

Objective

We propose to develop a microfluidic chip-sized assay for low-cost, sensitive, rapid, and multiplexed detection of bacteria in urine. We aim to reduce bacterial diagnostic time from the current standard of 1-3 days to several minutes. The device will have application to both conventional clinical microbiology laboratories and point-of-care settings, and will eliminate dependence on highly trained operators. We propose to use isotachophoresis (ITP) to extract and concentrate the bacterial signature 16S ribosomal RNA directly from urine samples, and detect and quantify its presence using a set of complementary fluorescent probes (molecular beacons) on a low-cost microfluidic chip.

Fields of science

• natural sciences › physical sciences › classical mechanics › fluid mechanics › microfluidics
• natural sciences › biological sciences › microbiology › bacteriology
• medical and health sciences › clinical medicine › urology

Programme(s)

• FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)

Topic(s)

• FP7-PEOPLE-2011-CIG - Marie-Curie Action: "Career Integration Grants"

Call for proposal

FP7-PEOPLE-2011-CIG
See other projects for this call

Funding Scheme

MC-CIG - Support for training and career development of researcher (CIG)

Coordinator

TECHNION - ISRAEL INSTITUTE OF TECHNOLOGY

Address

Senate Building Technion City
32000 Haifa
Israel

Activity type

Higher or Secondary Education Establishments

Administrative Contact

Mark Davison (Mr.)

Links

EU contribution

€ 100 000