Objective
Immunological reactions represent one of the most feared adverse drug events; they cannot be predicted and show no simple dose-response relationship. The presence of drug-response T-lymphocytes in patients with cutaneous reactions provides a robust case for their involvement in the disease pathogenesis. In contrast, the role of T-cells in reactions targeting the liver is less well defined.
We have recently defined the profile of drug protein conjugation at specific amino acid residues with respect to dose and incubation time and characterized the minimum levels of modification associated with the stimulation of T-cells. We now aim to utilize these methods to characterize the cellular response in patients with flucloxacillin-induced liver injury. Drug antigens will be characterized by mass spectrometry and used to define the phenotype (CD, homing receptors) and function (Th1, Th2, Th17 & cytotoxicity) of antigen-specific T-lymphocytes and T-cell clones.
Recently, an association between expression of HLA-B*5701 and flucloxacillin-induced liver injury has been described, which implies a direct effect of the gene product on the disease pathogenesis. To explore whether susceptibility relates to the restriction of the fit of the drug antigen in MHC, we will utilize our unique cell bank of HLA-typed healthy volunteers and a novel in vitro assay to relate phenotype to function and to investigate the additional signals that might be required to convert an antigenic signal into an aberrant T-cell response.
The Fellow will be trained in bioanalytical and cell culture methods that are directly applicable to immunological drug reactions. Furthermore, the Liverpool CDSS and the Ajou School of Medicine will establish a collaboration to transfer clinical samples to investigate other HLA associations in different ethnic groups. This will allow us to respond in real-time to newly identified genetic variants associated with adverse reactions
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences chemical sciences organic chemistry amines
- natural sciences chemical sciences analytical chemistry mass spectrometry
- medical and health sciences basic medicine pharmacology and pharmacy adverse drug reactions
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2011-IIF
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
L69 7ZX LIVERPOOL
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.