Objective
During early mouse embryogenesis the cells of the blastocyst’s inner cell mass take a lineage decision to contribute either to the epiblast or the primitive endoderm (PE). The allocation of cells to either lineage depends on the activity of FGF signaling and two gene regulatory networks (GRNs), one centered on the transcription factor Nanog, the other one relying on Gata factors. The two GRNs are initially activated in an overlapping and heterogeneous pattern in the ICM, and have been proposed to compete each other out over time. The dynamics of this competition, and how the transition state between the two lineages, marked by co-expression Gatas and Nanog, is resolved, is not known.
Here I propose to address these questions in vitro by recapitulating the competition between the Gata- and Nanog-GRNs through the controlled overexpression of Gata factors. This converts embryonic stem cells (ESCs), which contribute primarily to the epiblast when introduced in chimeras, into extraembryonic endoderm (XEN) cells, which contribute solely to PE derivatives. I will combine fluorescent reporters with this ES-to-XEN transition to ask with which dynamics transitions occur in individual cells, whether they involve heterogeneities at the population level, and how these parameters are controlled by the activity of gene regulatory networks and signaling pathways. I will aim at identifying culture conditions that stabilize the transition state, where cells might be on the brink of being XEN, and therefore akin to ICM. I hypothesize, that under these conditions cells will be endowed with higher developmental potential compared to parental ES cells, and be able to contribute to both epiblast and PE-derived tissues.
The results of this project will enhance our understanding of the mechanisms underlying lineage decisions in early development and may uncover more general principles that govern the way in which differentiating cells are specified in a stem cell pool.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences developmental biology
- medical and health sciences medical biotechnology cells technologies stem cells
- medical and health sciences clinical medicine embryology
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2011-IEF
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
CB2 1TN CAMBRIDGE
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.