Objectif Current interest in neural stem cells derives from the prospect of using them in brain repair strategies, but also to understand neurodevelopmental pathologies. This will require, however, a significant improvement of our understanding of the gene expression programs associated with their maintenance and differentiation, and how these are regulated. Recently, several lines of evidence suggest a regulatory function for the zinc-finger transcription factor ZEB1, both in embryonic neural stem/progenitor cells, and in human tumours of neural origin. Here we propose to use a multidisciplinary approach, combining mouse genetics and genomics, to characterize the function of ZEB1 in neural development. A thorough phenotypic analysis of ZEB1 loss-of-function mouse models will be performed, with a special focus on its role in neural progenitors. This will be complemented with the genome-wide characterization of its transcriptional network, using a combination of genomic location analysis with expression profiling. Moreover, a detailed comparison of the ZEB1 transcriptional programs in human foetal- and glioma-derived stem cells will be carried out, providing important insights into ZEB1 function in tumour initiation and development. Champ scientifique natural sciencesbiological sciencesneurobiologynatural sciencesbiological sciencesgeneticsmedical and health sciencesmedical biotechnologycells technologiesstem cellsmedical and health sciencesbasic medicinepathology Programme(s) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Thème(s) FP7-PEOPLE-2011-CIG - Marie-Curie Action: "Career Integration Grants" Appel à propositions FP7-PEOPLE-2011-CIG Voir d’autres projets de cet appel Régime de financement MC-CIG - Support for training and career development of researcher (CIG) Coordinateur FUNDACAO CALOUSTE GULBENKIAN Contribution de l’UE € 100 000,00 Adresse AVENIDA BERNA 45A 1067-001 LISBOA Portugal Voir sur la carte Région Continente Área Metropolitana de Lisboa Área Metropolitana de Lisboa Type d’activité Research Organisations Contact administratif José Mario Leite (Mr.) Liens Contacter l’organisation Opens in new window Site web Opens in new window Coût total Aucune donnée
