Do plants go further in deciding their cell fate: different target genes, different Polycomb Group mechanisms?

Projektinformationen

PLANTPCGSILENCING

ID Finanzhilfevereinbarung: 333748

Abgeschlossenes Projekt

Startdatum 1 April 2013

Enddatum 31 März 2017

Finanziert unter

• Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)

Gesamtkosten € 100 000,00

EU-Beitrag € 100 000,00

Koordiniert durch

AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
Spain

Ziel

The epigenetic mechanisms that control chromatin organization are known to regulate cell differentiation in both animals and plants. Nevertheless, why plants, unlike animals, so readily switch their cell fate in response to external signals is unclear. Furthermore, how plants regenerate a complete organism from a single somatic cell remains a mystery. Post-translational histone modifications contribute to the maintenance of chromatin states associated with a defined gene expression program. Among histone modifiers, the evolutionary conserved Polycomb Group (PcG) machinery has a key role in repressing the genes that are not required in a specific cell fate, thus, establishing cell identity. Unfortunately, our knowledge of this important regulatory mechanism is still incomplete, especially in plants in which only some PcG proteins are known. Thus far, plant PRC2-like complexes are relatively well studied, but the existence of plant PRC1 components has just been discovered. These components are homologs to animal PRC1 Ring finger proteins and are involved in H2A monoubiquitination. Plants impaired in PRC1 Ring finger components undergo dedifferentiation of somatic cells into embryonic cells, indicating that they play a central role in maintaining cell identity. Interestingly, the PRC1 Ring finger proteins apparently regulate only a subset of PRC2 targets; moreover, there are different plant-specific proteins that participate in the regulation of specific targets, suggesting that not all PcG targets are regulated by the same mechanism. Our main goal is to unveil the different PcG regulatory mechanisms in plants and their biological functions. Using a combination of genetic and biochemical approaches, we will purify the putative PRC1 complexes, determine the direct targets of the PRC1 Ring finger proteins and unveil the role of H2A monoubiquitination in the mechanism. These findings will shed light on important aspect of plant epigenetics and developmental biology.

Wissenschaftliches Gebiet

• Naturwissenschaften › Biowissenschaften › Biochemie › Biomoleküle › Proteine
• Naturwissenschaften › Biowissenschaften › Entwicklungsbiologie
• Naturwissenschaften › Biowissenschaften › Genetik › Epigenetik

Programm/Programme

• FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013)

Thema/Themen

• FP7-PEOPLE-2012-CIG - Marie-Curie Action: "Career Integration Grants"

Aufforderung zur Vorschlagseinreichung

FP7-PEOPLE-2012-CIG
Andere Projekte für diesen Aufruf anzeigen

Finanzierungsplan

MC-CIG - Support for training and career development of researcher (CIG)

Koordinator

AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS

Kontakt Verwaltung

Alberto Sereno Alvarez (Mr.)

• Adresse: Calle serrano 117 ; 28006 Madrid ; Spanien
• Region: Comunidad de Madrid › Comunidad de Madrid › Madrid
• Aktivitätstyp: Research Organisations
• EU-Beitrag: Keine Daten