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Non-invasive nanotransducer for in vivo gene therapy

Objective

Nanotechnology is expected to have a major impact on biomedical research, leading to new types of diagnostic and therapeutic tools. One focus in nanobiotechnology is the development of non-viral vectors for safe and efficient gene delivery. Progress in the Human Genome Project increases the attractiveness of gene therapy as a therapeutic modality for a host of diseases. Today the methodologies for in vivo gene transfection are still in their infancy. The most promising method is based on the use of viral vectors, but its safety is actually under discussion.

This project aims at developing a nonviral vector for gene delivery, able of:
a) gene transfection in vivo and on a large amount of cells,
b) local and non invasive therapy,
c) frequent and easy medication.

This nano-device will be based on a carbon nanotube (CNT). It will be coated with genes to be transferred, and will be functionalised with a ligand, able to bind a receptor expressed by the target cells to be transfected. A solution containing a myriad of vectors will be dispensed locally in the target tissue, e.g. via injection; each of them will localise a target cell and bind it. Two strategies of cell transfection will be investigated: 1) cells up -take the vectors via endocytosis and 2) genes are transferred in the cell membrane via electroporation (i.e. via permeabilization of cell membrane by application of short-duration electric field pulses).

In the second case, current impulses could be generated in CNTs via electromagnetic fields, by exploiting t he properties of CNTs like antennas. In order to prove these paradigms, in vivo validation of the nanotransducer vectors will be performed. A specific neurological disorder will be treated to demonstrate their therapeutic potential. The implementation of t his proposed solution requires a multidisciplinary approach and the integration of expertise in the fields of nanotechnologies, biotechnologies, communication technologies and neuroscience.

Call for proposal

FP6-2004-NMP-TI-4
See other projects for this call

Funding Scheme

STREP - Specific Targeted Research Project

Coordinator

SCUOLA SUPERIORE DI STUDI UNIVERSITARI E DI PERFEZIONAMENTO SANT'ANNA
Address
Piazza Martiri Della Liberta' 33'
Pisa
Italy

Participants (5)

THE SCHOOL OF PHARMACY, UNIVERSITY OF LONDON
United Kingdom
Address
Brunswick Square 29-39
London
MAX PLANCK GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V.
Germany
Address
Hofgartenstrasse 8
Muenchen
CONSIGLIO NAZIONALE DELLE RICERCHE
Italy
Address
Piazzale Aldo Moro 7
Roma
THALES SA
France
Address
Rue De Villiers 45
Neuilly Sur Seine
NANOTHINX SA
Greece
Address
Stadiou Street, Platani
Rio Patras