Final Report Summary - SIGNALING 3D (Three Dimensional Single Cell Analysis of the Cancer Stem Cell Inducing Epithelial-Mesenchymal Transition Signaling Networks in Breast Cancer by Mass Cytometry)
To study cell-to-cell communication and the cellular activation of EMT in breast cancer tissues, we developed a new imaging technology that we termed imaging mass cytometry. In this technology, a laser ablation system is coupled to a mass cytometer and 50 markers can be imaged at subcellular resolution on cancer tissues in 2D and 3D. To analyze highly multiplexed tissue images, we have developed a novel data analysis toolbox, which we called histoCAT. Using these imaging approaches, we have analyzed hundreds of breast cancer patient samples to generate a comprehensive map of the single-cell pathology and processes of breast cancer and reveal EMT in a subset of breast cancer patients. In summary, we have developed a novel tissue imaging technology and used single-cell systems biology analysis to understand the regulatory circuits that govern breast cancer signaling networks and to map of the single-cell pathology of breast cancer.