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Receptors, Channels and Transporters:
Development and Application of Novel Technologies for Structure Determination

Objective

Structure determination of G protein-coupled receptors (GPCRs) has been exceedingly successful over the last 5 years due to the development of complimentary generic methodologies that will now allow the structure determination of virtually any GPCR. However, these technologies address only two aspects of the process, namely the stability of the receptors during purification and the ability to form well-diffracting crystals. The strategies also apply only to GPCRs and not transporters or ion channels. The recent successes have been of GPCRs that are expressed in either yeasts or in insect cells using the baculovirus expression system, but many membrane proteins are expressed poorly in these systems or may be expressed in a misfolded non-functional form. A second issue with the future structure determination of GPCRs is the lack of generic technologies to allow the crystallisation of arrestin-GPCR and G protein-GPCR complexes. Although one G protein GPCR complex has been crystallised this was exceedingly diffciult and resulted in poor resolution of the GPCR component of the complex. We believe that it is possible to thermostabilise both arrestin and heterotrimeric G proteins, which will allow a simplified strategy for the crystallisation and structure determination of GPCR complexes. This is based on the development of the strategy of conformational thermostabilisation of GPCRs developed in our lab that has resulted in the structure determination of 3 different GPCRs bound to either antagonists, partial agonists, full agonists and/or biased agonists.

The aims are:
1. The development of generic methodology for the production of eukaryotic membrane proteins in mammalian cells.
2. The development of a thermostable functional arrestin mutant
3. Structures of β1-adrenoceptor, adenosine A2A receptor and angiotensin receptor bound to a G protein and arrestin
4. Understanding the role of each amino acid residue in the activation process of GPCRs through saturation mutagenes

Field of science

  • /natural sciences/chemical sciences/organic chemistry/amines
  • /engineering and technology/materials engineering/crystals
  • /natural sciences/biological sciences/biochemistry/biomolecules/proteins

Call for proposal

ERC-2013-ADG
See other projects for this call

Funding Scheme

ERC-AG - ERC Advanced Grant

Host institution

MEDICAL RESEARCH COUNCIL
Address
North Star Avenue Polaris House 2 Floor David Phillips Building
SN2 1FL Swindon
United Kingdom
Activity type
Research Organisations
EU contribution
€ 2 378 162
Principal investigator
Christopher Gordon Tate (Dr.)
Administrative Contact
Lisa Fields (Ms.)

Beneficiaries (1)

MEDICAL RESEARCH COUNCIL
United Kingdom
EU contribution
€ 2 378 162
Address
North Star Avenue Polaris House 2 Floor David Phillips Building
SN2 1FL Swindon
Activity type
Research Organisations
Principal investigator
Christopher Gordon Tate (Dr.)
Administrative Contact
Lisa Fields (Ms.)