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Training Network on novel animal models for medical purposes

Objectif

The overall goal is to develop a complex training program for PhD students on mammalian developmental biology, embryology, stem cell research combined with transgenic technology, genetic reprogramming, laboratory animal science and appropriate micromanipulation, microscopic imaging and molecular biological methods, using real-time PCR, micro-arrays andproteomics. Such training would reduce the fragmentation in the ERA in these areas and would enable the graduates to tackle the complexity of transgenic model and genetic reprogramming research. The research rationale of the project is that model organisms are essential to study the genetic basis of human diseases. Transgenic models, especially genetic knock-out mice catalysed the progress. To continue the advan cement, further sophisticated and refined models are crucially needed to study the genetic basis and manifestations of numerous human diseases. This proposal is aimed to develop new tools for the generation of transgenic laboratory animal models both in mo use and rat for comparative analyses of functional genomics. In the mouse stable embryonic stem cell lines enable gene targeting. However, the lack of such cell lines in most mammalians has so far prevented targeted genetic modifications. This proposal is aimed to use new tools such as nuclear replacement and gene targeting in somatic cells as a technological platform for producing new transgenic models. The aim is to further explore and validate the nuclear replacement system for safety and reliability as a technological platform. Our goal is also to generate the first gene targeted rat models. Relevant issues, such as the role of mitochondrial inheritance, somatic cell gene targeting and standardisation of new genotypes by cryo-preservation will be addresse d. Generation of embryonic stem cell lines from cloned embryos and use of embryonic stem cells for partial reprogramming of somatic cells will be studied, as well.

Champ scientifique (EuroSciVoc)

Mots‑clés

Thème(s)

Appel à propositions

FP6-2005-MOBILITY-1
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Régime de financement

RTN - Marie Curie actions-Research Training Networks

Coordinateur

MEZOGAZDASAGI BIOTECHNOLOGIAI KUTATOKOZPONT
Contribution de l’UE
Aucune donnée
Coût total
Aucune donnée

Participants (11)