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Content archived on 2024-05-29

Development of 3D in vitro models of estrogen-reporter mouse tissues for the pharmaco-toxicological analysis of nuclear receptors-interacting compounds (NR-ICs)

Objective

Stakes: Availability of adequate test model systems is essential to the development of risk assessment programmes for nuclear receptors-interacting compounds (NR-ICs) (drugs, industrial compounds, cosmetics, environmental contaminants and food components).
Goal: The objective of the EXERA project is to develop novel 3D in vitro models of mouse tissues from five major organs for the pharmaco-toxicological analysis of Estrogen Receptors-Interacting Compounds (ER-ICs): liver, skin and bone (non reproductive systems), ovary and testis (male and female reproductive systems).
Work content: This objective will be pursued through a Work Programme which allies an integrated scientific approach between innovative technologies such as the 3D-culture device, known as "Rotary Cell Culture System" (RCCS Technology adapted to the needs of this project), established transgenic mouse lines (estrogen-reporter mice, here called MOUSE-1) and genomic platforms for ER-ICs characterization. Our strategy is planned around 5 main points including, besides the mentioned technologies, multiple cell cultures quality controls, immortalisation control, cell banking and the use of specific markers of estrogenic action and cell differentiation/health, in order to obtain estrogen-responsive 3D-cultures of well differentiated mouse cells.
Expected Results and Related Benefits: The corresponding work programme will be ensured by an important involvement of the whole partnership and by a high degree of coordination between 7 private and 3 public institutions.
The expected results of our effort will be scientifically, socially and economically relevant:
1) application/adaptation of new 3D-culture technologies to cell cultures devoted to the study of ER-ICs;
2) constitution of a cell bank and, finally
3) a battery of differentiated 3D cell-based systems derived from estrogen-reporter mice for basic and applied research (e.g. pharmacology and toxicology), public use and industrial use.

Fields of science (EuroSciVoc)

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Topic(s)

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Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

FP6-2005-LIFESCIHEALTH-7
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Funding Scheme

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STREP - Specific Targeted Research Project

Coordinator

AZIENDA OSPEDALIERA- SPEDALI CIVILI DI BRESCIA
EU contribution
No data
Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

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Participants (8)

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