Objective
The partners will develop methods and instruments to analyze the copy number of nucleic acid sequences down to the
single cell/single molecule level, with the goal of developing an early diagnosis system for a child disease, namely
hemophagocytic lymphohistiocytosis (HLH). The underlying technique is amplification based counting (ABC), enabling to
quantify the copy number of genetic sequences with a resolution of about 100 base pairs in single cells. ABC is based on
the discovery that there is a strong correlation between the number of positive PCRs from a genetic sequence and the
sequence copy number if the sequence is present only in a few copies per sample. The method provides a resolution
which is much higher than for Fluorescence In Situ Hybridization and works quantitatively with lower sample amounts than
quantitative PCR. To prove ABC's effectiveness for clinical applications the partners will develop a single cell manipulation
unit that picks cells from a solution and transfers them onto an integrated PCR and hybridization slide (AmpliGrid). The
AmpliGrid contains dried-on PCR reagents as well as hybridization probes to detect the presence and specificity of the
PCR products. The single cells on the AmpliGrid will then be processed automatically in an integrated PCR and
hybridization machine (AmpliHyb). Clinical samples will be investigated in which copy number deviations are pathologic
as in genetic diseases. As a model system QuAGSiC chose HLH which is hard to diagnose and fatal without specific
therapeutic measures, as well as trisomy 21 which is relevant in prenatal and postnatal diagnostics. For these applications
the project will cover the complete workflow from identifying the cells of interest to quantifying the copy number of nucleic
acid sequences in clinical samples. Those diseases were selected as they will enable to demonstrate the efficiency of the
proposed diagnostic assay as well as its broad range of applications.'
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2005-LIFESCIHEALTH-7
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
EVRY
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.