Objective
The amyloid diseases, including for instance Alzheimer and Parkinson's diseases, are being actively investigated in recent years by biophysicists, biochemists, or neuroscientists. Despite of this, the fibrillogenesis mechanisms and the early events of fibril formation are still not well understood. Nonetheless, they are crucial for a better understanding and future improvements in healthcare particularly since there is growing evidence that it is the small oligomers that cause cell death. Therefore these early stages will be the focus of the current work. We will merge, in a multidisciplinary proposal, some standard techniques used in the amyloid field, such as light scattering or electron microscopy, and the novel single-molecule fluorescence technique of two-colour coincidence detection (TCCD).
The TCCD technique allows the sensitive detection and quantification of the self-assembly of two or more molecules, each labelled with one of two different fluorophores. Therefore, this technique provides unique and valuable information on the aggregation kinetics, different oligomer size, and relative populations of oligomers allowing the determination of the energy landscape for oligomerisation. In a first step, we will characterize the early aggregation of a model peptide system, a Transthyretin 11-aminoacids fragment (TTR 105-115). The following phases will be to elucidate oligomerization of some full-length proteins: the SH3 domain of the p85-alpha subunit of phosphatidylinositol 3-kinase (PI3-SH3), and the alpha -synuclein. The later is the pathogenic protein in Parkinson's disease.
The last goal will be to examine the influence of two classes of protein chaperone on the early aggregation of alpha-synuclein. The chaperones will be the protein HSP70 and the alpha-crystallin. The biomedical relevance of these systems and the unique information that the TCCD technique provides make the current proposal an important advance in the amyloid fibrils field.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences basic medicine neurology dementia alzheimer
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences physical sciences optics microscopy electron microscopy
- medical and health sciences basic medicine neurology parkinson
- natural sciences physical sciences optics laser physics
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2005-MOBILITY-5
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
CAMBRIDGE
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.