Objectif The European Community produces every year 200 million tonnes of biodegradable wastes, which have to be diverted from land-filling according to European laws. Biological digestion, either aerobic or anaerobic, is the most environmentally favourable option f or the recycling of biowastes. Composts resulting from these processes are used as soil improvers, which, among other benefits, exert plant disease supressiveness due to the presence of microorganisms that are antagonists to pathogens. However, improperly treated composts may spread pathogenic microorganisms, thus damaging the environment and posing risks to human health. This causes concern among the general public, especially after the food alerts during the last decade, and constitutes a priority issue within the European Research Area.Therefore, a tool that allows the quick analysis of compost microbiota is needed to safeguarde its agronomic value. DNA biochip technology offers the possibility to simultaneously detect an entire array of micro-organisms. The host group has recently designed a micro-array addressed towards compost microbiota. This proposal aims to design an oligonucleotide micro-array to detect signature microorganisms in composts from anaerobic digestion. Micro-organisms that will be isolated from compost will be targeted. Additional probes will be selected on basis of sequences determined from bands in DGGE gels run with compost DNA. Oligonucleotide probes will be designed using sequence differences in the 16S rDNA gene and printed onto the arrays.Fluorescently labelled DNA will be prepared and hybridised, and the arrays analysed by fluorescent screening. The validation of the micro-array will be mainly conducted through spiking experiments and through their wide application to already characterised compost samples. If successful, the micro-array might permit assessing whether soil improvers meet hygiene demands, thus increasing their marketability and promoting confidence among European consumers Champ scientifique ingénierie et technologiebiotechnologie environnementalebioremédiationbioréacteursciences naturellessciences biologiquesmicrobiologiebactériologiesciences naturellessciences biologiquesgénétiqueADNingénierie et technologiebiotechnologie environnementalebioremédiationcompostsciences naturellessciences chimiqueschimie organiquecomposés aliphatiques Mots‑clés Anaerobic Digestion Compost DNA biochips Disease supressive microorganisms Food safety Pathogenic microorganisms Programme(s) FP6-MOBILITY - Human resources and Mobility in the specific programme for research, technological development and demonstration "Structuring the European Research Area" under the Sixth Framework Programme 2002-2006 Thème(s) MOBILITY-2.1 - Marie Curie Intra-European Fellowships (EIF) Appel à propositions FP6-2005-MOBILITY-5 Voir d’autres projets de cet appel Régime de financement EIF - Marie Curie actions-Intra-European Fellowships Coordinateur LEOPOLD-FRANZENS-UNIVERSITÄT INNSBRUCK Adresse Innrain 52 Innsbruck Autriche Voir sur la carte Liens Site web Opens in new window Contribution de l’UE Aucune donnée
