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CORDIS - Forschungsergebnisse der EU
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Developing a spectroscopic system to detect dysplasia in real time and non-invasively

Final Activity Report Summary - CANCERSCANNER (Developing a spectroscopic system to detect dysplasia in real time and non-invasively)

The goal of the project was developing a spectroscopic system to detect cancerous tissue in real-time and non-invasively. We have developed a spectroscopy system named 'Elastic light single-scattering spectroscopy' (ELSSS) to perform ex-vivo and in-vivo experiments on animal models and human. ELSSS consists of a miniature spectrometer, a tungsten halogen white light source, a single-fibre optical probe, and a laptop computer. A single-fibre optical probe was used for both delivery and detection of white light to the tissue and from the tissue. We have performed in-vivo experiments on an animal model and in-vivo and ex-vivo experiments on human to differentiate cancerous tissue from non-cancerous tissue non-invasively and in-real time. The ELSSS system is portable, in-expensive and safe for patients. Amount of the light delivered to the patients is less than 2 mW, and does not give any damage to patients. The results of the researches conducted during the project are summarised below.

We have investigated the potential application of ELSSS as an adjunctive tool for intra-operative rapid detection of brain tumours and demarcation of the tumour from the surrounding normal tissue. Our results indicate that signs of spectral slopes may enable the discrimination of brain tumours from surrounding normal white matter brain tissue with a sensitivity of 93% and specificity of 100%. The research has been published.

In vivo experiments have been conducted on 20 lesions from 18 patients (11 men with mean age of 68 and 7 women with mean age of 52) applied to the Department of Dermatology and Venerology. Before the biopsy, spectra are taken on the lesion and adjacent (approximately 1 cm distant) normal-appearing skin. Spectra of the normal skin are used as a control group. The spectra are correlated to the pathology results with sensitivity and specificity of 82% and 89%, respectively. Due to small diameter of fibre probe and limited number of sampling (15), some positive cases are missed, which is lowered the sensitivity of the system. The results are promising and could suggest that the system may be able to detect malignant skin lesion non-invasively and in real time.

ELSSS system developed and tested performing in-vivo and ex-vivo experiments on animal and human tissues. Only one single-fibre optical probe was used to deliver and detect light to and from tissues in these studies. Since the diameter of the single-fibre optical probes was 100 micro meter, inspecting the complete tissue requires long time and is not clinically suitable. Therefore, we have constructed an array of 7x7 single-fibre optical probes to scan a 25 mm2 tissue surface systematically with a resolution of 0.7 mm within one minute. The new system is consists of two optical switches of 1x50, multi fibre optical probe, spectrometer, and a computer. The system has been successfully tested performing in-vitro experiments taking spectra from a tissue phantom (dispersed microsphere polystyrene particles in water). The next step is conducting in-vivo experiments to detect cervical dysplasia and skin malignancies and discriminate brain tumour from normal brain white matter.