Final Activity Report Summary - RESPOLMED (Bioresorbable Polymers for Medical Applications)
Investigation of the target graft copolymers by Size Exclusion Chromatography and NMR spectroscopy showed the presence of some uncapped PLA homopolymer (not attached to an oxanorbornyl group), produced as a side product during the ROP of lactide. Our investigation showed that the presence of PLA homopolymer impurity in the target graft copolymers significantly increases the rate of degradation of the final material. Therefore, we developed a convenient procedure of graft copolymers purification to remove PLA homopolymer from the samples.
The target graft copolymers were subjected to degradation in phosphate buffer solution. The degradation studies at two different temperatures, 37C and 50C showed that, as expected, increasing the temperature significantly increased the rate of degradation. Comparison of degradation behaviour of graft copolymers with the same oxanorbornyl backbone and different length of PLA grafts indicated that samples with shorter PLA grafts degrade more slowly. The results also indicated that target graft copolymers with one PLA side chain exhibit fastest rate of degradation. One explanation is that the presence of two PLA side chains on each five membered ring in the backbone chain induces steric hindrance and therefore reduces the rate of degradation.
The materials based on pure PLA homopolymer show 80% weight loss in about 80 days whereas the weight loss of our target graft copolymers is 40% over the same time. This clearly demonstrates that the rate of degradation of the PLA is greatly reduced by attaching them (grafting) to a polyoxanorbornene backbone chain. This concept of retardation of degradation was the main objective of this work which has been successfully achieved. The medical device industry as a whole can benefit from the outcome of the research and synthesis of a new class of materials from which to fashion devices for patient benefit.