Objective The present understanding of cellular signal transduction is restricted, at the best, to the wiring schemes of signalling pathways. Little is known about the details of their dynamic operation and the importance of quantitative, spatial and time-dependent parameters for signalling output. Those are, however, crucially important for drug discovery and application. QUASI is a multidisciplinary project with the goal to obtain a coherent and detailed picture of the dynamic operation of a model signalling transduction network. The signalling pathways contain the evolutionary conserved MAP kinase cascade module, which is of central importance for signalling in human cells and implicated in human diseases such as cancer and inflammatory disorders. MAP kinase pathways are currently being explored as drug targets. A better understanding of the dynamic operation of these pathways offers new opportunities for drug discovery and for efficient individualised treatment based on the genetic setup of the patient (pharmacogenomics). To achieve the goals of QUASI, quantitative data of high definition on signal transduction activation and deactivation will be obtained using frontline experimental approaches encompassing global gene expression, proteomics, bioimaging and chemical genetics. A software-implemented mathematical model of signalling dynamics will be constructed from pre-existing data and data generated within the consortium. Predictions from the model will be used as a basis for experimentation to further enhance the model, in a recursive manner. An interactive dynamic visual interface will be constructed to allow the experimenter to explore the effects of virtual manipulations of the system. This tool will enhance the intuitive understanding of intracellular signalling and this interface could be developed into a general tool for education as well as prediction of drug effects. Fields of science natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsproteomicsnatural sciencesbiological sciencescell biologycell signalingnatural sciencesbiological sciencesbiochemistrybiomoleculeslipidsnatural scienceschemical sciencesanalytical chemistrymass spectrometrynatural sciencesmathematicsapplied mathematicsmathematical model Keywords MAP kinases Signal transduction cellular dynamics mathematical models Programme(s) FP6-LIFESCIHEALTH - Life sciences, genomics and biotechnology for health: Thematic Priority 1 under the Focusing and Integrating Community Research programme 2002-2006. Topic(s) LSH-2002-1.1.0-1 - Topics for Specific Targeted Research Project/CA in the area of Fundamental knowledge and basic tools for functional genomics in all organisms Call for proposal FP6-2002-LIFESCIHEALTH See other projects for this call Funding Scheme STREP - Specific Targeted Research Project Coordinator GOETEBORG UNIVERSITET EU contribution No data Address Vasaparken 100 GOETEBORG Sweden See on map Total cost No data Participants (5) Sort alphabetically Sort by EU Contribution Expand all Collapse all UNIVERSITAT POMPEU FABRA Spain EU contribution No data Address ¨Placa de la Merce, 12 BARCELONA See on map Total cost No data INSTITUT FUER BIOCHEMIE UND MOLEKULARE ZELLBIOLOGIE DER UNIVERSITAET WIEN Austria EU contribution No data Address Dr. Bohrgasse 9 WIEN See on map Total cost No data EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH Switzerland EU contribution No data Address Raemistrasse 101 ZURICH See on map Total cost No data MAX PLANCK GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V. Germany EU contribution No data Address Hofgartenstrasse 8 101062 MUENCHEN See on map Total cost No data MAELARDALENS HOEGSKOLA Sweden EU contribution No data Address SANKTA URSULAS VAEG 2A 883 VAESTERAAS See on map Total cost No data
